Protein Name & Synonyms:
Epidermal growth factor receptor (EC 22.214.171.124) (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1)
Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
1 mg/ml in 1X PBS, BSA free, sodium azide free
Human EGFR purified from A431 cells was used as the immunogen for the anti-EGFR antibody.
Protein G affinity chromatography
Human. Other species not tested.
Mouse IgG1, kappa
This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.