Mouse anti-Human EGFR Antibody [Sodium Azide Free]

Mouse anti-Human EGFR Antibody [Sodium Azide Free]


Antigen Information

Accession Number:
  • P00533
Gene ID:
  • 1956
Gene Symbols:
  • EGFR
  • ERBB
  • ERBB1
  • HER1
Protein Name & Synonyms:
Epidermal growth factor receptor (EC (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1)
Target Species:
  • Human

Antibody Specifications

Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
1 mg/ml in 1X PBS, BSA free, sodium azide free
Cell surface
Host Species:
Human EGFR purified from A431 cells was used as the immunogen for the anti-EGFR antibody.
Protein G affinity chromatography
Species Reactivity:
Human. Other species not tested.
Mouse IgG1, kappa


This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.


The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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