Human EGFR Phosphorylation Array C1

RayBio® C-Series Human EGFR Phosphorylation Antibody Array 1 Kit. Detects 16 site specific and pan Human EGFRs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$686.60  
AAH-PER-1-8
+

Assay Format

Pathway:
  • HER/ErbB Signaling
Number of Targets Detected:
16
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
  • EGFR
  • ERBB2
  • ERBB3
  • ERBB4

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
EGFR
(P-Tyr845)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Tyr992)
EGFR
(P-Tyr1045)
EGFR
(P-Tyr1068)
EGFR
(P-Tyr1086)
EGFR
(P-Tyr1148)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Tyr1173)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EGFR
(P-Ser1046/1047)
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
ErbB2
(P-Tyr877)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr1112)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr 1221/1222)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Tyr1248)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Thr686)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB2
(P-Ser1113)
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB3
(P-Tyr1289)
  • Gene Symbol: ErbB3
  • Gene ID: 2065
  • Accession #: P21860
ErbB4
(P-Tyr1284)
  • Gene Symbol: ErbB4
  • Gene ID: 2066
  • Accession #: Q15303
       

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human EGFR Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail Set I
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Zhang J., et al. YAP-dependent induction of amphiregulin identifies a non-cell-autonomous component of the Hippo pathway. Nat Cell Biol. 2009 Dec;11(12):1444-50. doi: 10.1038/ncb1993.
    Species: Human
    Sample type: Cell Lysate (MCF10A cell lines transfected with agent)
  2. Zhu S., et al. DARPP-32 Increases Interactions Between Epidermal Growth Factor Receptor and ERBB3 to Promote Tumor Resistance to Gefitinib. Gastroenterology. 2011 Nov;141(5):1738-48.e1-2. doi: 10.1053/j.gastro.2011.06.070.
    Species: Human
    Sample type: Cell Lysate (MKN-28 gastric cancer cell lines)
  3. Bhuvaneswari R., et al. Targeting EGFR with photodynamic therapy in combination with Erbitux enhances in vivo bladder tumor response., Mol Cancer. 2009 Nov 2;8:94. doi: 10.1186/1476-4598-8-94.
    Species: Human
    Sample type: Cell Lysate (MGH bladder cancer cells)
  4. Wang X., Zhang M., Ping F., et al. Identification and therapeutic intervention of coactivated anaplastic lymphoma kinase, fibroblast growth factor receptor 2, and ephrin type-A receptor 5 kinases in hepatocellular carcinoma. Hepatology. 2018 Jan 21. doi: 10.1002/hep.29792.
    Species: Human
    Sample type: Cell Lysate (Heptocellular carnoma cell lines SMMC-7721 and others)
  5. Ruiz-Saenz A., Dreyer C., Campbell M., et al. HER2 amplification in tumors activates PI3K/Akt signaling independent of HER3. Cancer Research, epub online 2018, doi: 10.1158/0008-5472.CAN-18-0430
    Species: Human
    Sample type: Cell Lysate (Breast cancer model of Her2 signaling importance)
  6. Panupinthu N., et al. Self-reinforcing loop of amphiregulin and Y-box binding protein-1 contributes to poor outcomes in ovarian cancer. Oncogene (2014) 33, 2846-2856; doi:10.1038/onc.2013.259
    Species: Human
    Sample type: Cell Lysate (CAOV3 Cells)
  7. Shalaby A., et al. The role of epidermal growth factor receptor in chordoma pathogenesis: a potential therapeutic target. J Pathol. 2011 Feb;223(3):336-46. doi: 10.1002/path.2818.
    Species: Human
    Sample type: Cell Lysate (U-CH1 chordoma cell line)
  8. Shalaby A., et al. The role of epidermal growth factor receptor in chordoma pathogenesis: a potential therapeutic target. J Pathol. 2011 Feb;223(3):336-46. doi: 10.1002/path.2818.
    Species: Human
    Sample type: Tissue Lysate (Chordoma lysate)
  9. Park JE., Hypoxic Tumor Cell Modulates Its Microenvironment to Enhance Angiogenic and Metastatic Potential by Secretion of Proteins and Exosomes. Molecular & Cellular Proteomics, June 1, 9, 1085-1099.
    Species: Human
    Sample type: Cell Lysate
  10. Kesanakurti D., et al. Functional cooperativity by direct interaction between PAK4 and MMP-2 in the regulation of anoikis resistance, migration and invasion in glioma. Cell Death and Disease (2012) 3, e445; doi:10.1038/cddis.2012.182
    Species: Human
    Sample type: Cell Lysate (Glioma Tumor lysate)
ErbB Array was used to test expression in whole cell lysates under differential drug conditions. Nice to have many phospho-site test spots for EGFFR and HER2, but only one each for HER3 and HER4. Many control (positive and negative) spots were helpful as well. Would be easier for data analysis if serial dilution spots (top, left three) were designated more specifically on protocol. Overall very helpful product!

MGH
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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