Human Fractalkine ELISA

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RayBio® Human Fractalkine (CX3CL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-Fractalkine-1
+

Antigen Information

Accession Number:
  • P78423
Gene ID:
  • 6376
Gene Symbols:
  • A-152E5.2
  • CX3CL1
  • FKN
  • NTT
  • SCYD1
Protein Name & Synonyms:
Fractalkine (C-X3-C motif chemokine 1) (CX3C membrane-anchored chemokine) (Neurotactin) (Small-inducible cytokine D1) [Cleaved into: Processed fractalkine]
Target Species:
  • Human

Assay Format

Detection Range:
300 pg/ml - 60.00 ng/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
300 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Unal S., Ozdemir O., Ozcimen AA., Oztas Y. Increase of serum fractalkine and fractalkine gene expression levels in sickle cell disease patients. Int J Hematol. 2015 Feb;101(2):114-8. doi: 10.1007/s12185-014-1718-4.
    Species: Human
    Sample type: Serum
  2. You YW., Qin YQ., Lin X., et al. Inhibition of Lipopolysaccharide-Induced Expression of Fractalkine by Methylprednisolone via NF-?b in Human Renal Tubular Epithelial Cells. J Pulm Respir Med 5:249. doi:10.4172/2161-105X.1000249
    Species: Human
    Sample type: Conditioned Media
  3. Szukiewicz D., Wojciechowska M., Bilska A., et al. Aspirin Action in Endothelial Cells: Different Patterns of Response Between Chemokine CX3CL1/CX3CR1 and TNF-?/TNFR1 Signaling Pathways. Cardiovasc Drugs Ther. 2015 Jun;29(3):219-29. doi: 10.1007/s10557-015-6589-2.
    Species: Human
    Sample type: Conditioned Media
  4. GARCIA, JUAN J.  and  ORTEGA, EDUARDO. Soluble fractalkine in the plasma of fibromyalgia patients. An. Acad. Bras. Ciênc. [online]. 2014, vol.86, n.4, pp. 1915-1917.  Epub Dec 09, 2014. ISSN 0001-3765.
    Species: Human
    Sample type: Conditioned Media
  5. Sczukiewicz D., Kochanowski J., Mittal T., Pyzlak M., Szewczyk G., Cendrowski K. (ChA) modifies CX3CL1 (fractalkine) production by human amniotic epithelialcells (HAEC) under normoxic and hypoxic conditions. Journal of Inflammation 2014, 11:12
    Species: Human
    Sample type: Cell Lysate
  6. Ceyla I., Tuba T., Adisen E., et al. Fractalkine (CX3CL1) levels in patients with Behçet's disease and neuro-Behçet's disease. J Neurol Sci. 2012 Apr 15;315(1-2):120-2. doi: 10.1016/j.jns.2011.11.011.
    Species: Human
    Sample type: Serum
  7. Yadav, Ashok Kumar, Anupam Lal, and Vivekanand Jha. "Association of circulating fractalkine (CX3CL1) and CX3CR1+ CD4+ T cells with common carotid artery intima-media thickness in patients with chronic kidney disease." Journal of atherosclerosis and thrombosis 18.11 (2011): 958-965.
    Species: Human
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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