Human Growth Factor Array C1

RayBio® C-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.

AAH-GF-1-2
+

Antigen Information

Gene Symbols:
AREG | CSF1 | CSF1R | CSF2 | CSF3 | EGF | EGFR | FGF2 | FGF4 | FGF6   View more

Assay Format

Number of Targets Detected:
41
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Amphiregulin
bFGF
beta-NGF
EGF
EGFR
FGF-4
FGF-6
FGF-7
(KGF)
GCSF
GDNF
GM-CSF
HB-EGF
HGF
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
M-CSF
M-CSF R
NT-3
NT-4
PDGF R alpha
PDGF R beta
PDGF-AA
PDGF-AB
PDGF-BB
PLGF
SCF
SCF R
(CD117/c-kit)
TGF alpha
TGF beta 1
TGF beta 2
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Growth Factor Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Zheng Y., Ji S., Wu H., et al. Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment. Biomaterials. 2017 Jan;113:56-67. doi: 10.1016/j.biomaterials.2016.10.031.
    Species: Human
    Sample type: Conditioned Media
  2. Chan J., Tan M., Sng M., et al. Cancer-associated fibroblasts enact field cancerization by promoting extratumoral oxidative stress. Cell Death Dis 8 (1), e2562. 2017 Jan 19.
    Species: Human
    Sample type: Conditioned Media
  3. Oltolina F., Zamperone A., Colangelo D., et al. Human Cardiac Progenitor Spheroids Exhibit Enhanced Engraftment Potential. LoS One. 2015 Sep 16;10(9):e0137999. doi: 10.1371/journal.pone.0137999
    Species: Human
    Sample type: Cell Lysate
  4. Kim E. Choi J., Kim J., et al. Injectable and Thermosensitive Soluble Extracellular Matrix and Methylcellulose Hydrogels for Stem Cell Delivery in Skin Wounds. Biomacromolecules. 2016 Jan 11;17(1):4-11. doi: 10.1021/acs.biomac.5b01566
    Species: Human
    Sample type: Conditioned Media
  5. Pan, Xiao-Ping, et al. "Efficient generation of functional hepatocyte-like cells from mouse liver progenitor cells via indirect co-culture with immortalized human hepatic stellate cells." Hepatobiliary & Pancreatic Diseases International 15.2 (2016): 173-179.
    Species: Human
    Sample type: Conditioned Media
  6. Trian T., Allard B., Dupin I., et al. House dust mites induce proliferation of severe asthmatic smooth muscle cells via an epithelium-dependent pathway. Am J Respir Crit Care Med. 2015 Mar 1;191(5):538-46. doi: 10.1164/rccm.201409-1582OC
    Species: Human
    Sample type: Conditioned Media
  7. Pickard A, McDade SS, McFarland M, McCluggage WG, Wheeler CM, McCance DJ (2015) HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion in Organotypic Cultures. PLoS Pathog 11(6): e1004988. doi:10.1371/journal.ppat.1004988
    Species: Human
    Sample type: Conditioned Media
  8. Sawada K., Takedachi M., Yamamoto S., et al. Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament cells. Biochem Biophys Res Commun. 2015 Aug 14;464(1):299-305. doi: 10.1016/j.bbrc.2015.06.147
    Species: Human
    Sample type: Conditioned Media
  9. Fessler E., Borovski T., Medema J. Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF. Mol Cancer. 2015 Aug 19;14:157. doi: 10.1186/s12943-015-0420-3
    Species: Human
    Sample type: Conditioned Media
  10. Chou Y., Young T., Lou P. Acta Biomater. Effects of biomaterial-derived fibroblast conditioned medium on the ?-amylase expression of parotid gland acinar cells. 2015 Nov;27:214-23. doi: 10.1016/j.actbio.2015.08.050.
    Species: Human
    Sample type: Conditioned Media

No posts found

Write A Review
U.S. Non-U.S.

Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.