Human Growth Factor Array C1

RayBio® C-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.

$616.50  
AAH-GF-1-4
+

Assay Format

Number of Targets Detected:
41
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
AREG | CSF1 | CSF1R | CSF2 | CSF3 | EGF | EGFR | FGF2 | FGF4 | FGF6   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Amphiregulin
bFGF
beta-NGF
EGF
EGFR
FGF-4
FGF-6
FGF-7
(KGF)
GCSF
GDNF
GM-CSF
HB-EGF
HGF
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
M-CSF
M-CSF R
NT-3
NT-4
PDGF R alpha
PDGF R beta
PDGF-AA
PDGF-AB
PDGF-BB
PLGF
SCF
SCF R
(CD117/c-kit)
TGF alpha
TGF beta 1
TGF beta 2
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
VEGF-A
VEGFR2
VEGFR3
VEGF-D

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Growth Factor Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Trian T., Allard B., Dupin I., et al. House dust mites induce proliferation of severe asthmatic smooth muscle cells via an epithelium-dependent pathway. Am J Respir Crit Care Med. 2015 Mar 1;191(5):538-46. doi: 10.1164/rccm.201409-1582OC
    Species: Human
    Sample type: Conditioned Media (Bronchial epithelial cells)
  2. Fessler E., Borovski T., Medema J. Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF. Mol Cancer. 2015 Aug 19;14:157. doi: 10.1186/s12943-015-0420-3
    Species: Human
    Sample type: Conditioned Media (Endothelial cell conditioned media with cancer stem cells)
  3. Al-aidaroos A., et al. Metastasis-associated PRL-3 induces EGFR activation and addiction in cancer cells. J Clin Invest. 2013;123(8):3459-3471. doi:10.1172/JCI66824.
    Species: Human
    Sample type: Cell Lysate (A2780 Ovarian Cancer Cells and A431 cells)
  4. Traktuev D, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks. Circulation Research. 2008; 102: 77-85
    Species: Human
    Sample type: Conditioned Media (Conditioned Media)
  5. Lee EJ., Xu L., Kim GH., et al. Regeneration of peripheral nerves by transplanted sphere of human mesenchymal stem cells derived from embryonic stem cells. Biomaterials. 2012 Oct;33(29):7039-46. doi: 10.1016/j.biomaterials.2012.06.047
    Species: Human
    Sample type: Conditioned Media (Mesenchymal Stem Cells)
  6. Zheng Y., Ji S., Wu H., et al. Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment. Biomaterials. 2017 Jan;113:56-67. doi: 10.1016/j.biomaterials.2016.10.031.
    Species: Human
    Sample type: Conditioned Media (Living Micronized Amniotic Membrane)
  7. Choe, G., Kim, S., Park, J., Park, J., Kim, S., Kim, Y. S., … Lee, J. Y. (2019). Anti-oxidant activity reinforced reduced graphene oxide/alginate microgels: Mesenchymal stem cell encapsulation and regeneration of infarcted hearts. Biomaterials, 119513. doi:10.1016/j.biomaterials.2019.119513 
    Species: Human
    Sample type: Conditioned media (Cell culture media)
  8. De S, Razorenova O, McCabe NP, O'Toole T, Qin J, Byzova TV. VEGF-integrin interplay controls tumor growth and vascularization. Proc Natl Acad Sci USA. 2005;102(21):7589-7594.
    Species: Mouse
    Sample type: Conditioned Media (Prostate tumor Cell-conditioned media supernates)
  9. Okuda H, Kobayashi A, Xia B, et al. Hyaluronan synthase HAS2 promotes tumor progression in bone by stimulating the interaction of breast cancer stem-like cells with macrophages and stromal cells. Cancer Research 2012;72(2):537-547. doi:10.1158/0008-5472.CAN-11-1678.
    Species: Human
    Sample type: Conditioned Media (Tumor associated Macs and Cancer stem cells)
  10. Okuda H., Kobayashi A., Xia B., Watabe M., et al. Hyaluronan Synthase HAS2 Promotes Tumor Progression in Bone by Stimulating the Interaction of Breast Cancer Stem-Like Cells with Macrophages and Stromal Cells. Cancer Research. January 15, 2012 72; 537
    Species: Human
    Sample type: Conditioned Media (Tumor Associated Macrophages Cancer stem-like cells)
We have utilized this kit to evaluate the growth factor components from the human adipose derived mesenchymal cell secretome (https://www.sciencedirect.com/science/article/pii/S2452199X21005508). The assay was sensitive and easy to perform and the results were reliable. We really like this product.

University of Nebraska Medical Center
A high throughput assay can give you 42 GFs levels in your testest sample within 48 hrs. Easy to do and read the dots.

Cleveland Clinic
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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