RayBio® Human HGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Assay Format

Detection Range:
3 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
3 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P14210
Gene ID:
  • 3082
Gene Symbols:
  • HGF
  • HPTA
Protein Name & Synonyms:
Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. De Barros, Sandra, et al. "Aging-related decrease of human ASC angiogenic potential is reversed by hypoxia preconditioning through ROS production." Molecular Therapy 21.2 (2013): 399-408.
    Species: Human
    Sample type: Conditioned Media (hASCs under normoxic and hypoxic conditions)
  2. ?ahin, Nur, et al. "The levels of hepatocyte growth factor in serum and follicular fluid and the expression of c-Met in granulosa cells in patients with polycystic ovary syndrome." Fertility and sterility 99.1 (2013): 264-269.
    Species: Human
    Sample type: Serum (PCOS patients undergoing IVF)
  3. ?ahin, Nur, et al. "The levels of hepatocyte growth factor in serum and follicular fluid and the expression of c-Met in granulosa cells in patients with polycystic ovary syndrome." Fertility and sterility 99.1 (2013): 264-269.
    Species: Human
    Sample type: Follicular Fluid (PCOS patients undergoing IVF)
  4. Nazarov I, Lee JW, Soupene E, et al. Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. Stem Cells Translational Medicine. 2012;1(5):359-372. doi:10.5966/sctm.2011-0021.
    Species: Human
    Sample type: Conditioned Media ( human chorionic mesenchymal stem cell derived from placenta MSCs derived from human bone marrow)
  5. Ali Jalili, Neeta Shirvaikar, Leah A. Marquez-Curtis, A. Robert Turner, and Anna Janowska-Wieczorek. Stem Cells and Development. August 2010, 19(8): 1143-1151. doi:10.1089/scd.2009.0376.
    Species: Human
    Sample type: Plasma
  6. The HGF/c-Met Axis Synergizes with G-CSF in the Mobilization of Hematopoietic Stem/Progenitor Cells
    Species: Human
    Sample type: Conditioned Media (mobilized peripheral blood cells, bone marrow-derived CD34+ hematopoietic cells, leukocytes )
  7. Shintani, Yusuke, et al. "Hepatocyte growth factor promotes an anti-inflammatory cytokine profile in human abdominal aortic aneurysm tissue." Atherosclerosis 216.2 (2011): 307-312.
    Species: Human
    Sample type: Conditioned Media
  8. Drebert Z., Bracke M., Beck I. Glucocorticoids and the non-steroidal selective glucocorticoid receptor modulator, compound A, differentially affect colon cancer-derived myofibroblasts. J Steroid Biochem Mol Biol. 2015 May;149:92-105. doi: 10.1016/j.jsbmb.2015.02.002.
    Species: Human
    Sample type: Conditioned Media (CT5.hTERT myofibroblasts )
  9. Stewart MD., Ramani VC., Sanderson RD. Shed Syndecan-1 Translocates to the Nucleus of Cells Delivering Growth Factors and Inhibiting Histone Acetylation: A NOVEL MECHANISM OF TUMOR-HOST CROSS-TALK. mJ Biol Chem. 2015 Jan 9;290(2):941-9. doi: 10.1074/jbc.M114.608455.
    Species: Human
    Sample type: Conditioned Media (CAG myeloma cells)
  10. Jung W., Han S., Kim S., Kim M., Lee J., Seo K., Youn H., Lee H. Stimulatory Effect of HGF-Overexpressing Adipose Tissue-Derived Mesenchymal Stem Cells on Thymus Regeneration in a Rat Thymus Involution Model. Cell Biology International Accepted May 7 2014,
    Species: Human
    Sample type: Conditioned Media (Human Adipose Tissue Mesenchymal Stem Cell supernatant)

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