Human HSP Array C1

RayBio® C-Series Human Heat Shock Protein Antibody Array Kit. Detects 9 Human heat shock proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$631.05  
AAH-HSP-1-8
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Assay Format

Number of Targets Detected:
9
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
  • DNAJB5
  • GH1
  • HMOX1
  • HSP90AA1
  • HSPA14
  • HSPA4
  • HSPB1
  • HSPE1
  • RPS27A

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
HSP10
  • Gene Symbol: HSPE1
  • Gene ID: 3336
  • Accession #: P61604
HSP27
HSP32
  • Gene Symbol: HMOX1
  • Gene ID: 3162
  • Accession #: P09601
HSP40
  • Gene Symbol: DNAJB5
  • Gene ID: 25822
  • Accession #: O75953
HSP60
  • Gene Symbol: HSPA14
  • Gene ID: 51182
  • Accession #: Q0VDF9
HSP70
HSP90
GRP75
  • Gene Symbol: GH1
  • Gene ID: 2688
  • Accession #: P01241
Ubiquitin+1

Heat shock protein profiling in induced HepG2 cultured cells

Heat Shock Protein Profiling

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human HSP Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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