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Antigen Information
Accession Number:
- P01579
Gene ID:
- 3458
Gene Symbol:
- IFNG
Protein Name & Synonyms:
Interferon gamma (IFN-gamma) (Immune interferon)
Target Species:
- Human
Assay Format
Detection Range:
15 pg/ml - 15000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
15 pg/ml
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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Plasma
- Serum
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
Species: Human
Sample type: Plasma -
Sharm S., Shiman P., Rajendiran S., et al. Osteoarticular tuberculosis: in search of new biomarkers. European Orthopaedics and Traumatology. September 2015, Volume 6, Issue 3, pp 195-200
Species: Human
Sample type: Serum -
Moore Q., Smith C., Wolf S., et al. Examining the immune response of pneumolysin exposed human corneal epithelial cells in a keratitis model. BIOS 85(4):199-206. 2014. doi: http://dx.doi.org/10.1893/0005-3155-85.4.199
Species: Human
Sample type: Conditioned Media -
Shi L., Chen S., Zha X., et al. Enhancement of the TCR? Expression, Polyclonal Expansion, and Activation of T Cells from Patients with Acute Myeloid Leukemia After IL-2, IL-7, and IL-12 Induction. DNA Cell Biol. 2015 Jul;34(7):481-8. doi: 10.1089/dna.2015.2810.
Species: Human
Sample type: Conditioned Media -
Zaki M., Foad M., El-Deeb D., El-Kheir M. Diagnostic Performance of Gamma Interferon in Rapid Diagnosis of Tuberculosis Peritonitis. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 6 (2015) pp. 1-13
Species: Human
Sample type: Peritoneal Fluid -
Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
Species: Human
Sample type: Conditioned Media -
Yang Z., Lei Y., Chen C., et al. Roles of the programmed cell death 1, T cell immunoglobulin mucin-3, and cluster of differentiation 288 pathways in the low reactivity of invariant natural killer T cells after chronic hepatitis B virus infection. Archives of Virology October 2015, Volume 160, Issue 10, pp 2535-2545
Species: Human
Sample type: Conditioned Media -
Prandota J., Elleboudy N., Ismail K., et al. Increased Seroprevalence of Chronic Toxoplasmosis in Autistic Children: Special Reference to the Pathophysiology of IFN-? and NO Overproduction. Int. J. of Neurol. Res. 2015 September 1(3): 102-122. doi:10.17554/j.issn.2313-5611.2015.01.30
Species: Human
Sample type: Serum -
Abbas, H., Ali, K. et al. Comparison study between typhoid fever and tuberculosis patients to induceand produce of Th-1 cytokines (IL-18 and IFN-?). European Journal of Experimental Biology, 2014, 4(3):20-26
Species: Human
Sample type: Serum -
Abdallah M., et al. Serum Th1/Th2 and Macrophage Lineage Cytokines in Leprosy; Correlation with Circulating CD4+ CD25highFoxP3+ T-regs Cells. Experimental Dermatology, August 2014 Accepted Article, doi:10.1111/exd.12529
Species: Human
Sample type: Serum
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
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