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Human IFN-gamma ELISA

RayBio® Human IFN-gamma ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$402.00  
ELH-IFNg-1
+ −
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Antigen Information

Accession Number:
  • P01579
Gene ID:
  • 3458
Gene Symbols:
  • IFNG
Protein Name & Synonyms:
Interferon gamma (IFN-gamma) (Immune interferon)
Target Species:
  • Human

Assay Format

Detection Range:
15 pg/ml - 15000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
15 pg/ml

Need more sensitivity? Check out the new IQELISA&tra

Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
    Species: Human
    Sample type: Plasma
  2. Plant, Ewan P., et al. "Influenza virus NS1 protein mutations at position 171 impact innate interferon responses by respiratory epithelial cells."?Virus research?240 (2017): 81-86.
    Species: Human
    Sample type: cell supernatants
  3. Sharm S., Shiman P., Rajendiran S., et al. Osteoarticular tuberculosis: in search of new biomarkers. European Orthopaedics and Traumatology. September 2015, Volume 6, Issue 3, pp 195-200
    Species: Human
    Sample type: Serum
  4. Shi L., Chen S., Zha X., et al. Enhancement of the TCR? Expression, Polyclonal Expansion, and Activation of T Cells from Patients with Acute Myeloid Leukemia After IL-2, IL-7, and IL-12 Induction. DNA Cell Biol. 2015 Jul;34(7):481-8. doi: 10.1089/dna.2015.2810.
    Species: Human
    Sample type: Conditioned Media
  5. Zaki M., Foad M., El-Deeb D., El-Kheir M. Diagnostic Performance of Gamma Interferon in Rapid Diagnosis of Tuberculosis Peritonitis. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 4 Number 6 (2015) pp. 1-13
    Species: Human
    Sample type: Peritoneal Fluid
  6. Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
    Species: Human
    Sample type: Conditioned Media
  7. Yang Z., Lei Y., Chen C., et al. Roles of the programmed cell death 1, T cell immunoglobulin mucin-3, and cluster of differentiation 288 pathways in the low reactivity of invariant natural killer T cells after chronic hepatitis B virus infection. Archives of Virology October 2015, Volume 160, Issue 10, pp 2535-2545
    Species: Human
    Sample type: Conditioned Media
  8. Prandota J., Elleboudy N., Ismail K., et al. Increased Seroprevalence of Chronic Toxoplasmosis in Autistic Children: Special Reference to the Pathophysiology of IFN-? and NO Overproduction. Int. J. of Neurol. Res. 2015 September 1(3): 102-122. doi:10.17554/j.issn.2313-5611.2015.01.30
    Species: Human
    Sample type: Serum
  9. Moore Q., Smith C., Wolf S., et al. Examining the immune response of pneumolysin exposed human corneal epithelial cells in a keratitis model. BIOS 85(4):199-206. 2014. doi: http://dx.doi.org/10.1893/0005-3155-85.4.199
    Species: Human
    Sample type: Conditioned Media
  10. Abbas, H., Ali, K. et al. Comparison study between typhoid fever and tuberculosis patients to induceand produce of Th-1 cytokines (IL-18 and IFN-?). European Journal of Experimental Biology, 2014, 4(3):20-26
    Species: Human
    Sample type: Serum
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This human IFNg ELISA kit works very well. Steps included in the manufacturer's protocol were easy to follow and all the necessary materials were provided. The snap wells come in handy when running multiple experiments in small batches for optimization of the sample. We ran human plasma samples and the standards ran evenly and the detection concentration fell within the provided range which is rare for quantifying IFNg levels in human samples.
Shreya Deb
University of Illinois at Chicago 11/01/2020, 08:14 AM
This is the first time I've run an Interferon-g ELISA and I'm very happy with the results. I typically use the ELISpot platform and I'm happy that the results with this kit are far superior- very little background and fairly sensitive with the detection. I may try the newer version (with the qRT-PCR) readout for subsequent experiments to compare between kits.
Aish S
Mayo Clinic 01/02/2020, 06:14 PM
This human IFNg ELISA kit is good. Steps were clear and all necessary materials were provided. Since we ran multiple smaller experiments, snap wells were really handy. We used BAL from mice lungs and though the standard ran evenly, samples showed low IFNg levels.
Hesper Wong
University of Pittsburgh 07/24/2019, 12:11 PM
The human IFNg ELISA kit is easy to follow and reproducible. We used the kit with IFNg-secreting human cell lines following the manufacturer's protocol after the cells were treated with LPS. The supernatant was used to detect the IFNg. The kit worked perfectly when detecting high content of IFNg. However, when the IFNg content is low in the samples, more repeats may be needed as the sensitivity or accuracy of the kit is becoming low, too. Overall, the kit works well in detecting IFNg.
Rosa Ignacio 01/25/2018, 12:21 PM
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# Manual (Supernatants / Plasma / Serum)

# Manual (Lysates)

# SDS (Supernatants / Plasma / Serum)

# SDS (Lysates)

# Analysis Tool

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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