Human IGF-1 ELISA

RayBio® Human IGF-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,680.00  
ELH-IGF1-5
+

Assay Format

Detection Range:
0.1 ng/ml - 30 ng/ml
Recommended Dilution (serum & plasma):
2 - 20 fold
Sensitivity:
100 pg/ml

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Specificity:
This antibody pair can only detect a free form of human IGF-1. It does not detect a complex, such as binding with IGFBPs. This IGF-1 ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P05019
Gene ID:
  • 3479
Gene Symbols:
  • IBP1
  • IGF1
Protein Name & Synonyms:
Insulin-like growth factor I (IGF-I) (Mechano growth factor) (MGF) (Somatomedin-C)
Target Species:
  • Human

Target Background

Insulin-like-growth factor is a hormone structurally similar to insulin. It is central to the somatotropic axis acting downstream of growth hormone (GH) to promote tissue growth and development. IGF1 is synthesized in the liver, where its secretion is regulated by the pituitary secretion of GH. The actions of IGF1 are mediated through the IGF1R receptor to activate downstream signaling cascades such as PI3-kinase and mitogen-activated protein kinase pathways. Defects in the encoding gene can cause insulin-like growth factor deficiency - characterized by growth retardation, sensorineural deafness and intellectual disability.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Del Fante, Claudia, et al. "Conditioned Medium Originated From Lethally Irradiated Umbilical Cord Blood-Derived Mononuclear Cells Has Different Pro-Angiogenic Effects Over Mature and Progenitor Endothelial Cells In Vitro." (2013): 1068-1068.
    Species: Human
    Sample type: Conditioned Media (monnuclear cells)
  2. Pezzotti, Giuseppe, et al. "Silicon nitride surface chemistry: a potent regulator of mesenchymal progenitor cell activity in bone formation." Applied Materials Today 9 (2017): 82-95.
    Species: Human
    Sample type: Conditioned Media (bone marros stromal stem cells)
  3. Pezzotti G., Bock R., Adachi T., et al. Silicon nitride surface chemistry: A potent regulator of mesenchymal progenitor cell activity in bone formation. Applied Materials Today. Volume 9, December 2017, Pages 82-95
    Species: Human
    Sample type: Conditioned Media (KUSA-A1 cell culture supernates)
  4. Guillen M, Me??as J, Gomar F, Alcaraz MJ. Haem oxygenase-1 regulates catabolic and anabolic processes in osteoarthritic chondrocytes.J Pathol. 2008;214:515-522
    Species: Human
    Sample type: Conditioned Media (Ex Vivo Chondrocytes cell supernate)
  5. De Barros, Sandra, et al. "Aging-related decrease of human ASC angiogenic potential is reversed by hypoxia preconditioning through ROS production." Molecular Therapy 21.2 (2013): 399-408.
    Species: Human
    Sample type: Conditioned Media (hASCs under normoxic and hypoxic conditions)
  6. Kim S, Kang Y, Krueger CA, et al. Sequential delivery of BMP-2 and IGF-1 using a chitosan gel with gelatin microspheres enhances early osteoblastic differentiation. Acta Biomaterialia. 2012;8(5):1768-1777. doi:10.1016/j.actbio.2012.01.009.
    Species: Human
    Sample type: Conditioned Media (Microspheres loaded with IGF1)
  7. Pickard A, McDade SS, McFarland M, McCluggage WG, Wheeler CM, McCance DJ (2015) HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion in Organotypic Cultures. PLoS Pathog 11(6): e1004988. doi:10.1371/journal.ppat.1004988
    Species: Human
    Sample type: Conditioned Media (Conditioned form E6/7 Human foreskin keratinocytes)
  8. Vinayak, Shaveta, et al. "A clinical trial of lovastatin for modification of biomarkers associated with breast cancer risk." Breast cancer research and treatment 142.2 (2013): 389-398.
    Species: Human
    Sample type: Serum
  9. Dai Y, Ai B, Liu Y, Pascal LE, Wang Z, Dhir R, Sun X, Jiang Y. Abnormal expression of Rab27B in prostatic epithelial cells of benign prostatic hyperplasia alters intercellular communication. Int J Biochem Cell Biol. 2020 Dec 4;131:105898. doi: 10.1016/j.biocel.2020.105898. Epub ahead of print. PMID: 33285290.
    Species: Human
    Sample type: Conditioned Media (cell culture media)
  10. Stoppoloni D., et al. Effect of Glucosamine and its peptidyl-derivative on the production of extracellular matrix components by human primary chondrocytes. Osteoarthritis and Cartilage Sept 2014, epub ahead of print. http://dx.doi.org/10.1016/j.joca.2014.09.005
    Species: Human
    Sample type: Conditioned Media
Excellent to make an analysis of protein secretome in cell differentiation processes.

University of Puerto Rico
Thanks for having great customer service! I needed to find some more information about the detection and capture antibody and when I called they were so helpful and informative!

University of Florida
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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