Human IGFBP-1 ELISA

RayBio® Human IGFBP-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-IGFBP1-1
+

Antigen Information

Accession Number:
  • P08833
Gene ID:
  • 3484
Gene Symbols:
  • IBP1
  • IGFBP1
Protein Name & Synonyms:
Insulin-like growth factor-binding protein 1 (IBP-1) (IGF-binding protein 1) (IGFBP-1) (Placental protein 12) (PP12)
Target Species:
  • Human

Assay Format

Detection Range:
5 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
5 - 500 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Pineda M., Lu Z., Cao D., Kim J. Influence of Cancer-Associated Endometrial Stromal Cells on Hormone-Driven Endometrial Tumor Growth. Horm Cancer. 2015 Aug;6(4):131-41. doi: 10.1007/s12672-015-0223-4
    Species: Human
    Sample type: Conditioned Media
  2. Founds S., Zeng X., Lykins D., and Robers J.
    Species: Human
    Sample type: Serum
  3. Aucouturier J, Thivel D, Isacco L, et al. Combined food intake and exercise unmask different hormonal responses in lean and obese children. Appl Physiol Nutr Metab. 2013;38(6): 638-643
    Species: Human
    Sample type: Plasma
  4. Pflum ZE, Palumbo SL, Li W-J. Adverse effect of demineralized bone powder on osteogenesis of human mesenchymal stem cells. Exp Cell Res. 2013 Aug 1;319(13):1942-1955.
    Species: Human
    Sample type: Conditioned Media
  5. Wright, Jonathan L., et al. "A study of caloric restriction versus standard diet in overweight men with newly diagnosed prostate cancer: a randomized controlled trial." The Prostate73.12 (2013): 1345-1351.
    Species: Human
    Sample type: Serum
  6. Estella C, Herrer I, Moreno-Moya JM, et al. miRNA Signature and Dicer Requirement during Human Endometrial Stromal Decidualization In Vitro. Knott JG, ed. PLoS ONE. 2012;7(7):e41080. doi:10.1371/journal.pone.0041080.
    Species: Human
    Sample type: Conditioned Media
  7. Ammoun S., Schmid M., Zhou L., et al. Insulin-like growth factor-binding protein-1 (IGFBP-1) regulates human schwannoma proliferation, adhesion and survival. Oncogene. 2012 Mar 29;31(13):1710-22. doi: 10.1038/onc.2011.357
    Species: Human
    Sample type: Conditioned Media
  8. Colostrum Supplementation Restores Insulin-like Growth Factor -1 Levels and Alters Muscle Morphology Following Massive Small Bowel Resection
    Species: Human
    Sample type:
Serum
Very good result

NIH
Serum
Very good result

NIH
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.