Human IGFBP-2 ELISA

RayBio® Human IGFBP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-IGFBP2-1
+

Antigen Information

Accession Number:
  • P18065
Gene ID:
  • 3485
Gene Symbols:
  • BP2
  • IBP2
  • IGFBP2
Protein Name & Synonyms:
Insulin-like growth factor-binding protein 2 (IBP-2) (IGF-binding protein 2) (IGFBP-2)
Target Species:
  • Human

Assay Format

Detection Range:
20 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
200 - 2,000 fold
Sensitivity:
20 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, IGFBP-1, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample type: Serum
  2. Chen-Plotkin AS, Unger TL, Gallagher MD, et al. TMEM106B, the risk gene for frontotemporal dementia, is regulated by the miRNA-132/212 cluster and affects progranulin pathways. The Journal of neuroscience?: the official journal of the Society for Neuroscience 2012;32(33):11213-11227. doi:10.1523/JNEUROSCI.0521-12.2012.
    Species: Human
    Sample type: Conditioned Media
  3. Png K., Halberg N., Yoshida M., Tavazoie S. A microRNA regulon that mediates endothelial recruitment and metastasis by cancer cells. Nature. 2011 Dec 14;481(7380):190-4. doi: 10.1038/nature10661
    Species: Human
    Sample type: Conditioned Media
  4. Narayanan R., Fu B., Heald A., et al. IGFBP2 is a biomarker for predicting longitudinal deterioration in renal function in type 2 diabetes. Endocr Connect. 2012 Oct 24;1(2):95-102. doi: 10.1530/EC-12-0053.
    Species: Human
    Sample type: Plasma
  5. Dragoo J., Korotkova T., Wasterlain A., et al. Age-Related Changes of Chondrogenic Growth Factors in Platelet-Rich Plasma. Operative Techniques in Orthopaedics, Volume 22, Issue 2, June 2012, Pages 49-55
    Species: Human
    Sample type: Plasma
  6. Dokmanovic, Milos, et al. "Trastuzumab regulates IGFBP-2 and IGFBP-3 to mediate growth inhibition: implications for the development of predictive biomarkers for trastuzumab resistance." Molecular cancer therapeutics 10.6 (2011): 917-928.
    Species: Human
    Sample type: Conditioned Media
  7. Çelebi, Betül, Diego Mantovani, and Nicolas Pineault. "Irradiated mesenchymal stem cells improve the ex vivo expansion of hematopoietic progenitors by partly mimicking the bone marrow endosteal environment." Journal of immunological methods 370.1 (2011): 93-103.
    Species: Human
    Sample type: Conditioned Media
  8. Kühnl, Andrea, et al. "High expression of IGFBP2 is associated with chemoresistance in adult acute myeloid leukemia." Leukemia research 35.12 (2011): 1585-1590.
    Species: Human
    Sample type: Serum
  9. Tombolan, Lucia, et al. "High IGFBP2 expression correlates with tumor severity in pediatric rhabdomyosarcoma." The American journal of pathology 179.5 (2011): 2611-2624.
    Species: Human
    Sample type: Plasma
  10. Pereira-Fantini PM, et al., Colostrum Supplementation Restores Insulin-like Growth Factor -1 Levels and Alters Muscle Morphology Following Massive Small Bowel Resection. JPEN J Parenter Enteral Nutr. 2008 May-Jun;32(3):266-75. doi: 10.1177/0148607108316197.
    Species: Human
    Sample type:

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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