Human IGFBP-3 ELISA

RayBio® Human IGFBP-3 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$361.80
You save: $40.20 (10%)
ELH-IGFBP3-1
+

Antigen Information

Accession Number:
  • P17936
Gene ID:
  • 3486
Gene Symbols:
  • IBP3
  • IGFBP3
Protein Name & Synonyms:
Insulin-like growth factor-binding protein 3 (IBP-3) (IGF-binding protein 3) (IGFBP-3)
Target Species:
  • Human

Assay Format

Detection Range:
80 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
20 - 200 fold
Sensitivity:
80 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GMCSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-4, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1 alpha, TIMP-1, TIMP-2, TNFbeta, TGFbeta1, TGFbeta2, TGFbeta3, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Aucouturier J, Thivel D, Isacco L, et al. Combined food intake and exercise unmask different hormonal responses in lean and obese children. Appl Physiol Nutr Metab. 2013;38(6): 638-643
    Species: Human
    Sample type: Plasma
  2. Brosseau C, Pirianov G, Colston KW. Role of Insulin-Like Growth Factor Binding Protein-3 in 1, 25-Dihydroxyvitamin-D3-Induced Breast Cancer Cell Apoptosis. Int J Cell Biol. 2013;2013:960378.
    Species: Human
    Sample type: Conditioned Media
  3. Brosseau C, Pirianov G, Colston KW. Role of Insulin-Like Growth Factor Binding Protein-3 in 1, 25-Dihydroxyvitamin-D3-Induced Breast Cancer Cell Apoptosis. Int J Cell Biol. 2013;2013:960378.
    Species: Human
    Sample type: Cell Lysate
  4. Schlatzer DM, Sugalski JM, Chen Y, et al. Plasma proteome analysis reveals overlapping, yet distinct mechanisms of immune activation in chronic HCV and HIV infections. J Acquir Immune Defic Syndr. 2013 Mar 15; [Epub ahead of print] doi:10.1097/QAI.0b013e3182909847.
    Species: Human
    Sample type: Plasma
  5. Bachmayer, C., Kemmer, A., Ehrmann, N., Hasenberg, T., Lammert, A., & Hammes, H. P. (2013). Adipokines and endothelial dysfunction in obesity WHO° III. Microvascular research, 89, 129-133.
    Species: Human
    Sample type: Serum
  6. Brosseau, C., G. Pirianov, and K. W. Colston. "Role of insulin-like growth factor binding protein-3 in 1, 25-dihydroxyvitamin-D3-induced breast cancer cell apoptosis." International journal of cell biology 2013 (2013).
    Species: Human
    Sample type: Cell Lysate
  7. Bachmayer, C., et al. "Healthy obese and post bariatric patients–metabolic and vascular patterns." Experimental and Clinical Endocrinology & Diabetes 121.08 (2013): 483-487.
    Species: Human
    Sample type: Serum
  8. Dragoo J., Korotkova T., Wasterlain A., et al. Age-Related Changes of Chondrogenic Growth Factors in Platelet-Rich Plasma. Operative Techniques in Orthopaedics, Volume 22, Issue 2, June 2012, Pages 49-55
    Species: Human
    Sample type: Plasma
  9. Dokmanovic, Milos, et al. "Trastuzumab regulates IGFBP-2 and IGFBP-3 to mediate growth inhibition: implications for the development of predictive biomarkers for trastuzumab resistance." Molecular cancer therapeutics 10.6 (2011): 917-928.
    Species: Human
    Sample type: Conditioned Media
  10. Kim, Jin Soo, et al. "Inflammatory and tumor stimulating responses after laparoscopic sigmoidectomy." Yonsei medical journal 52.4 (2011): 635-642.
    Species: Human
    Sample type: Serum
The ELISA worked without a hitch. Easy to store, easy to use, and the sensitivity of the assay is better than those of even R&D Systems. The dilution that they recommended was also good for use in plasma.

Alkahest
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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