Human IgG1 ELISA

RayBio® Human IgG1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$361.80
You save: $40.20 (10%)
ELH-IGG1-1
+

Antigen Information

Accession Number:
  • P01857
Gene Symbols:
  • IGHG1
Protein Name & Synonyms:
Ig gamma-1 chain C region
Target Species:
  • Human

Assay Format

Detection Range:
0.05 ng/ml - 40 ng/ml
Recommended Dilution (serum & plasma):
~10,000,000 fold
Sensitivity:
50 pg/ml

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Specificity:
Detect human IgG1. No cross reactivity was found againt IgG2, IgG3, IgG4, IgM, IgA, IgD and IgE
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Olivares M., Castillejo G., Varea V., Sanz Y. Double-blind, randomised, placebo-controlled intervention trial to evaluate the effects of Bifidobacterium longum CECT 7347 in children with newly diagnosed coeliac disease. British Journal of Nutrition. DOI: http://dx.doi.org/10.1017/S0007114514000609 (About DOI), Published online: 28 April 2014. [Epub Ahead of Print]
    Species: Human
    Sample type: Serum
  2. Zhang B, Tang D, Goryacheva IY, et al. Anodic-stripping voltammetric immunoassay for ultrasensitive detection of low-abundance proteins using quantum dot aggregated hollow microspheres. Chemistry. 2013 Feb 11;19(7):2496-2503.
    Species: Human
    Sample type: Serum
  3. Zhang B, Tang D, Goryacheva IY, Niessner R, Knopp D. Anodic-Stripping Voltammetric Immunoassay for Ultrasensitive Detection of Low-Abundance Proteins Using Quantum Dot Aggregated Hollow Microspheres. Chemistry. 2013;19(7):2496-2503.
    Species: NA
    Sample type: N/A
  4. San Kim, Ho, et al. "Improvement of anti-Burkholderia mouse monoclonal antibody from various phage-displayed single-chain antibody libraries." Journal of immunological methods 372.1 (2011): 146-161.
    Species: Human
    Sample type: Conditioned Media
  5. Liao, En-Chih, and Jaw-Ji Tsai. "Clinical effectiveness of Tyrophagus putrescentiae allergy by local nasal immunotherapy using strips of Dermatophagoides pteronyssinus." Journal of Asthma 48.9 (2011): 957-964.
    Species: Human
    Sample type: Serum
  6. Prakash, S. S., et al. "Binding antibody responses to the immunogenic regions of viral envelope in hiv-1-infected indian children." Viral immunology 24.6 (2011): 463-469.
    Species: Human
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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