Human IL-1 beta ELISA

RayBio® Human IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

$1,608.00  
ELH-IL1b-5
+

Antigen Information

Accession Number:
  • P01584
Gene ID:
  • 3553
Gene Symbols:
  • IL1B
  • IL1F2
Protein Name & Synonyms:
Interleukin-1 beta (IL-1 beta) (Catabolin)
Target Species:
  • Human

Assay Format

Detection Range:
0.3 pg/ml - 100 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
0.3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
    Species: Human
    Sample type: Conditioned Media (HUVEC cells in vascular injury model)
  2. Tian, Wenfang, et al. "Brahma?related gene 1 bridges epigenetic regulation of proinflammatory cytokine production to steatohepatitis in mice." Hepatology58.2 (2013): 576-588.
    Species: Human
    Sample type: Conditioned Media (HepG2 cells )
  3. Šenolt, Ladislav, et al. "Adipokine profile is modulated in subcutaneous adipose tissue by TNF? inhibitors in patients with rheumatoid arthritis." Annals of the rheumatic diseases 70.11 (2011): 2054-2056.
    Species: Human
    Sample type: Tissue Lysate (adipose tissue)
  4. Chai, Q., Lu, Z., Liu, Z. et al. Lung gene expression signatures suggest pathogenic links and molecular markers for pulmonary tuberculosis, adenocarcinoma and sarcoidosis. Commun Biol 3, 604 (2020). https://doi.org/10.1038/s42003-020-01318-0
    Species: Human
    Sample type: Conditioned media (Cell culture media)
  5. Han Y, Xu X, Tang C, Gao P, Chen X, Xiong X, Yang M, Yang S, Zhu X, Yuan S, Liu F, Xiao L, Kanwar YS, Sun L. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biol. 2018 Jun;16:32-46. doi: 10.1016/j.redox.2018.02.013. Epub 2018 Feb 15. Erratum in: Redox Biol. 2019 Jun;24:101216. PMID: 29475133; PMCID: PMC5842313.
    Species: Human/mouse
    Sample type: (HK-2 Cells)
  6. Goetzl EJ, Schwartz JB, Abner EL, Jicha GA, Kapogiannis D. High complement levels in astrocyte-derived exosomes of Alzheimer disease. Ann Neurol. 2018 Mar;83(3):544-552. doi: 10.1002/ana.25172. Epub 2018 Mar 10. PMID: 29406582; PMCID: PMC5867263.
    Species: Human
    Sample type: Conditioned Media
  7. Piano S, Favaretto E, Tonon M, Antonelli G, Brocca A, Sticca A, Mareso S, Gringeri E, Scaroni C, Plebani M, Russo FP, Burra P, Cillo U, Angeli P. Including Relative Adrenal Insufficiency in Definition and Classification of Acute-on-Chronic Liver Failure. Clin Gastroenterol Hepatol. 2020 May;18(5):1188-1196.e3. doi: 10.1016/j.cgh.2019.09.035. Epub 2019 Oct 4. PMID: 31589973.
    Species: Human
    Sample type: Plasma, Saliva
  8. Unamuno X, Gómez-Ambrosi J, Ramírez B, Rodríguez A, Becerril S, Valentí V, Moncada R, Silva C, Salvador J, Frühbeck G, Catalán V. NLRP3 inflammasome blockade reduces adipose tissue inflammation and extracellular matrix remodeling. Cell Mol Immunol. 2019 Sep 24. doi: 10.1038/s41423-019-0296-z. Epub ahead of print. PMID: 31551515.
    Species: Human
    Sample type: Conditioned Media
  9. Ildefonso C., Jaime H., Biswal M., et al. Gene therapy with the caspase activation and recruitment domain reduces the ocular inflammatory response. Mol Ther. 2015 May;23(5):875-84. doi: 10.1038/mt.2015.30.
    Species: Human
    Sample type: Conditioned Media (Human HEK293T cells and ARPE-19 cells)
  10. Diabate M., Muron P., Garcia E., et al. Escherichia coli ?-hemolysin counteracts the anti-virulence innate immune response triggered by the Rho GTPase activating toxin CNF1 during bacteremia. PLoS Pathog. 2015 Mar 17;11(3):e1004732. doi: 10.1371/journal.ppat.1004732.
    Species: Human
    Sample type: Conditioned Media (Monocytes treated with CNF1 GTPase activating toxin)
This ELISA kit was used for quantification of IL-1 beta cytokine in the supernatants of peripheral blood mononuclear cells upon stimulation with genotoxics in a dose- and time dependent manner. The optical density (O.D.) values obtained were repetitive.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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