Human IL-10 ELISA

RayBio® Human IL-10 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$402.00  
ELH-IL10-1
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Antigen Information

Accession Number:
  • P22301
Gene ID:
  • 3586
Gene Symbols:
  • IL10
Protein Name & Synonyms:
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 150 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Attia EAS., et al. Serum Th17 cytokines in leprosy: correlation with circulatingCD4+ CD25highFoxP3+ T-regs cells, as well as down regulatory cytokines. Arch Dermatol Res. Published online July 2014. DOI 10.1007/s00403-014-1486-2
    Species: Human
    Sample type: Serum
  2. Chen Z., et al. IL-6, IL-10 and IL-13 are associated with pathogenesis in children with Enterovirus 71 infection. Int J Clin Exp Med 2014;7(9):2718-2723
    Species: Human
    Sample type: Serum
  3. Gao, S., Dine, J., et al. Anti-inflammatory and Anti-apoptotic Effect of Combined Treatment with Methylprednisolone and Amniotic Membrane Mesenchymal Stem Cells After Spinal Cord Injury in Rats. Neurochemical Research 2014. Epub Ahead of Print DOI:10.1007/s11064-014-1344-9
    Species: Human
    Sample type: Tissue Lysate
  4. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  5. Santarelli E., Gonnella R., Giovenale G., Cuomo L., Capobianchi A., et al. STAT3 activation by KSHV correlates with IL-10, IL-6 and IL-23 release and an autophagic block in dendritic cells. Scientific Reports, 2014.4 article 4241, doi:10.1038/srep04241
    Species: Human
    Sample type: Conditioned Media
  6. Wang, F., He, D., et al. Chemokine expression in diverse nonimmediate drug hypersensitivity reactions: focus on thymus activation-regulated chemokine, cutaneous T-cell-attracting chemokine, and interleukin-10. Annals of Allergy, Asthma & Immunology 2014. Epub Ahead of Print DOI: 10.1016/j.anai.2014.05.006
    Species: Human
    Sample type: Serum
  7. Chen J., Shen B., Jiang Y., Jun L., Zhu M., Chen B., Liu C. Analysis of immunoglobulin-like transcripts (ILTs) in lymphocytes with sHLA-G and IL10 from SLE patients. Clinical and Experimental Medicine, 2013. Vol 13; Issue 2, 135-142.
    Species: Human
    Sample type: Plasma
  8. Liou H-L, Shih C-C, Chung K-C, Chen HI. Comparison of the Effect of Intensive Versus Conventional Insulinotherapy in Patients with Cardiac Surgery after Cardiopulmonary Bypass. Chin J Physiol. 2013 Apr 30;56(2).
    Species: Human
    Sample type: Serum
  9. Rocher, Crystal, and Dinender K. Singla. "SMAD-PI3K-Akt-mTOR Pathway Mediates BMP-7 Polarization of Monocytes into M2 Macrophages." PLoS One. NCBI, 20 Dec. 2013. Web.
    Species: Human
    Sample type: Conditioned Media
  10. Koob, Thomas J., et al. "Biological properties of dehydrated human amnion/chorion composite graft: implications for chronic wound healing."?International wound journal?10.5 (2013): 493-500.
    Species: Human
    Sample type: Tissue Lysate
This kit consistently produced accurate results on our lab samples. I will buy more ELISA kits from RayBiotech in future.

University of Texas Dallas
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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