Human IL-10 ELISA

RayBio® Human IL-10 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$402.00  
ELH-IL10-1
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Antigen Information

Accession Number:
  • P22301
Gene ID:
  • 3586
Gene Symbols:
  • IL10
Protein Name & Synonyms:
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 150 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
    Species: Human
    Sample type: Conditioned Media (HUVEC cells in vascular injury model)
  2. Bose, Purabi Deka, et al. "High viral load and deregulation of the progesterone receptor signaling pathway: association with hepatitis E-related poor pregnancy outcome." Journal of hepatology 54.6 (2011): 1107-1113.
    Species: Human
    Sample type: Serum
  3. Chai, Q., Lu, Z., Liu, Z. et al. Lung gene expression signatures suggest pathogenic links and molecular markers for pulmonary tuberculosis, adenocarcinoma and sarcoidosis. Commun Biol 3, 604 (2020). https://doi.org/10.1038/s42003-020-01318-0
    Species: Human
    Sample type: Conditioned media (Cell culture media)
  4. Piano S, Favaretto E, Tonon M, Antonelli G, Brocca A, Sticca A, Mareso S, Gringeri E, Scaroni C, Plebani M, Russo FP, Burra P, Cillo U, Angeli P. Including Relative Adrenal Insufficiency in Definition and Classification of Acute-on-Chronic Liver Failure. Clin Gastroenterol Hepatol. 2020 May;18(5):1188-1196.e3. doi: 10.1016/j.cgh.2019.09.035. Epub 2019 Oct 4. PMID: 31589973.
    Species: Human
    Sample type: Plasma, Saliva
  5. Romanets-Korbut O., Kovalevska L., Seya T., et al. Measles virus hemagglutinin triggers intracellular signaling in CD150-expressing dendritic cells and inhibits immune response. Cellular & Molecular Immunology advance online publication 15 June 2015; doi: 10.1038/cmi.2015.55
    Species: Human
    Sample type: Conditioned Media (Dendritic cell conditioned media)
  6. Wang XS, Williams LA, Krishnan S, et al. Serum sTNFR1, IL6, and the Development of Fatigue in Patients with Gastrointestinal Cancer Undergoing Chemoradiation Therapy. Brain, Behavior, and Immunity. 2012;26(5):699-705. doi:10.1016/j.bbi.2011.12.007.
    Species: Human
    Sample type: Serum (Colorectal or esphageal cancer patients treated with concurrent chemoradiation therapy)
  7. Mankad D., Dupuis A., Smile S., et al. A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism. Mol Autism. 2015; 6: 18. Published online 2015 Mar 21. doi: 10.1186/s13229-015-0010-7
    Species: Human
    Sample type: Plasma (austistic children treatment with omega 3 fatty acids)
  8. Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.
    Species: Human
    Sample type: Conditioned Media (CD4 T cells with a TLR2 gene expression)
  9. Santarelli E., Gonnella R., Giovenale G., Cuomo L., Capobianchi A., et al. STAT3 activation by KSHV correlates with IL-10, IL-6 and IL-23 release and an autophagic block in dendritic cells. Scientific Reports, 2014.4 article 4241, doi:10.1038/srep04241
    Species: Human
    Sample type: Conditioned Media (Monocyte derived dendritic cell )
  10. Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
    Species: Human
    Sample type: Plasma (Diabetic Patient Samples)

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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