Human IL-15 ELISA

RayBio® Human IL-15 ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$756.00  
ELH-IL15-2
+

Assay Format

Detection Range:
3 pg/ml - 800 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P40933
Gene ID:
  • 3600
Gene Symbols:
  • IL15
Protein Name & Synonyms:
Interleukin-15 (IL-15)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Pfeiffer I., et al. Triggering of NF-?B in cytokine-matured human DCs generates superior DCs for T-cell priming in cancer immunotherapy. European Journal of Immunology. Accepted manuscript online: 6 AUG 2014 06:11PM EST | DOI: 10.1002/eji.201344417
    Species: Human
    Sample type: Conditioned Media
  2. Shen, Anding, et al. "Endothelial cell stimulation overcomes restriction and promotes productive and latent HIV-1 infection of resting CD4+ T cells." Journal of virology 87.17 (2013): 9768-9779.
    Species: Human
    Sample type: Conditioned Media (MCF7 cells - HIV-1 infection of resting CD4+ T cells)
  3. Gong X., et al. Insights into the paracrine effects of uterine natural killer cells. Molecular Medicine Reports10: 2851-2860, 2014. DOI: 10.3892/mmr.2014.2626
    Species: Human
    Sample type: Conditioned Media (NK secreted media)
  4. Modulation of the Expression of the Transcription Factors T-Bet and GATA-3 in Immortalized Human Endometrial Stromal Cells (HESCs) by Sex Steroid Hormones and cAMP
    Species: Human
    Sample type: Conditioned Media (conditioned media control cells vs. femal steroid hormone treated)
  5. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-?)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." Clinical rheumatology 30.1 (2011): 133-137.
    Species: Human
    Sample type: Serum
  6. Shoman S., et al. Assessment of immunological changes in Epstein-Barr virus co-infection in Egyptian chronic HCV patients. Memórias do Instituto Oswaldo Cruz. Epub ahead of print Aug 22, 2014 http://dx.doi.org/10.1590/0074-0276140049
    Species: Human
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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