Human IL-17 ELISA

RayBio® Human IL-17A ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • Q16552
Gene ID:
  • 3605
Gene Symbols:
  • CTLA8
  • IL17
  • IL17A
Protein Name & Synonyms:
Interleukin-17A (IL-17) (IL-17A) (Cytotoxic T-lymphocyte-associated antigen 8) (CTLA-8)
Target Species:
  • Human

Assay Format

Detection Range:
80 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
80 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Karadag AS., Ertugrul DT., Bilgil SG., et al. Immunoregulatory effects of isotretinoin in patients with acne. Br J Dermatol. 2012 Aug;167(2):433-5. doi: 10.1111/j.1365-2133.2012.10949.x.
    Species: Human
    Sample type: Serum
  2. Kurtuncu M., Tuzun E., Turkoglu R., et al. Effect of short-term interferon-? treatment on cytokines in multiple sclerosis: significant modulation of IL-17 and IL-23. Cytokine. 2012 Aug;59(2):400-2. doi: 10.1016/j.cyto.2012.05.004.
    Species: Human
    Sample type: Serum
  3. Ulusoy C., Tuzun E., Kurtuncu M., et al. Comparison of the cytokine profiles of patients with neuronal-antibody-associated central nervous system disorders. Int J Neurosci. 2012 Jun;122(6):284-9. doi: 10.3109/00207454.2011.648762.
    Species: Human
    Sample type: Serum
  4. Raychaudhuri S., Raychauduri S., Genovese M. IL-17 receptor and its functional significance in psoriatic arthritis. Mol Cell Biochem. 2012 Jan;359(1-2):419-29. doi: 10.1007/s11010-011-1036-6.
    Species: Human
    Sample type: Synovial Fluid
  5. Wolfram D., Rabensteiner E., Grundtman C., et al. T regulatory cells and TH17 cells in peri-silicone implant capsular fibrosis. Plast Reconstr Surg. 2012 Feb;129(2):327e-337e. doi: 10.1097/PRS.0b013e31823aeacf.
    Species: Human
    Sample type: Conditioned Media
  6. Li Y., Jiang L., Zhang S., et al. Methotrexate attenuates the Th17/IL-17 levels in peripheral blood mononuclear cells from healthy individuals and RA patients. Rheumatol Int. 2012 Aug;32(8):2415-22. doi: 10.1007/s00296-011-1867-1.
    Species: Human
    Sample type: Conditioned Media
  7. Sibilano R., Frossi B., Calvaruso M., et al. The aryl hydrocarbon receptor modulates acute and late mast cell responses. J Immunol. 2012 Jul 1;189(1):120-7. doi: 10.4049/jimmunol.1200009.
    Species: Human
    Sample type: Conditioned Media
  8. Kouam S., Kusari S., Lamshoft M., et al. Sapelenins G-J, acyclic triterpenoids with strong anti-inflammatory activities from the bark of the Cameroonian medicinal plant Entandrophragma cylindricum. Phytochemistry. 2012 Nov;83:79-86. doi: 10.1016/j.phytochem.2012.06.004.
    Species: Human
    Sample type: Conditioned Media
  9. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-?)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature."?Clinical rheumatology?30.1 (2011): 133-137.
    Species: Human
    Sample type: Serum
  10. Hakemi, Mazdak Ganjalikhani, et al. "Optimization of human Th17 cell differentiation in vitro: evaluating different polarizing factors."?In Vitro Cellular & Developmental Biology-Animal?47.8 (2011): 581.
    Species: Human
    Sample type: Conditioned Media

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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