Human IL-18 ELISA

RayBio® Human IL-18 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$864.00  
ELH-IL18-2
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Assay Format

Detection Range:
0.5 pg/ml - 75 pg/ml
Recommended Dilution (serum & plasma):
5 - 50 fold
Sensitivity:
0.5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q14116
Gene ID:
  • 3606
Gene Symbols:
  • IGIF
  • IL18
  • IL1F4
Protein Name & Synonyms:
Interleukin-18 (IL-18) (Iboctadekin) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma)
Target Species:
  • Human

Target Background

Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines. Similar to IL-1b, it is synthesized as an inactive precursor which requires processing by caspase-1. However, unlike IL-1b, IL-18 is constitutively active in nearly all healthy cells. IL-18 binding protein (IL-18BP) has high affinity for IL-18, thus mediating the activity of IL-18. IL-18 forms a signaling complex with the IL-18Rα receptor and IL-18Rβ co-receptor to mediate NF?B pro-inflammatory signaling. IL-18BP, present in the extracellular compartment, binds mature IL-18 preventing binding to the IL-18 receptor. IL-18 has been implicated in conditions such as autoimmune disease, myocardial infarction, metabolic syndromes, inflammatory bowel disease and psoriasis.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Pecorelli A, Cordone V, Messano N, Zhang C, Falone S, Amicarelli F, Hayek J, Valacchi G. Altered inflammasome machinery as a key player in the perpetuation of Rett syndrome oxinflammation. Redox Biol. 2020 Jan;28:101334. doi: 10.1016/j.redox.2019.101334. Epub 2019 Oct 6. PMID: 31606551; PMCID: PMC6812177.
    Species: Human
    Sample type: Serum
  2. Shiori Yoshikawa, Sachiyo Yoshio, Yuichi Yoshida, Yuriko Tsutsui, Hironari Kawai, Taiji Yamazoe, Taizo Mori, Yosuke Osawa, Masaya Sugiyama, Masashi Iwamoto, Koichi Watashi, Takumi Kawaguchi, Tomoyuki Akita, Junko Tanaka, Yoshimi Kikuchi, Masashi Mizokami, Shinichi Oka, Tatsuya Kanto, Hiroyuki Gatanaga, Impact of Immune Reconstitution-Induced Hepatic Flare on Hepatitis B Surface Antigen Loss in Hepatitis B Virus/Human Immunodeficiency Virus-1 Coinfected Patients, The Journal of Infectious Diseases, , jiaa662, https://doi.org/10.1093/infdis/jiaa662
    Species: Human
    Sample type: Serum
  3. Unamuno X, Gómez-Ambrosi J, Ramírez B, Rodríguez A, Becerril S, Valentí V, Moncada R, Silva C, Salvador J, Frühbeck G, Catalán V. NLRP3 inflammasome blockade reduces adipose tissue inflammation and extracellular matrix remodeling. Cell Mol Immunol. 2019 Sep 24. doi: 10.1038/s41423-019-0296-z. Epub ahead of print. PMID: 31551515.
    Species: Human
    Sample type: Conditioned Media
  4. Li X., Yan X., Wang Y., et al. NLRP3 inflammasome inhibition attenuates silica-induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells. Exp Cell Res. 2018 Jan 15;362(2):489-497. doi: 10.1016/j.yexcr.2017.12.013.
    Species: Human
    Sample type: Conditioned Media
  5. Han Y, Xu X, Tang C, Gao P, Chen X, Xiong X, Yang M, Yang S, Zhu X, Yuan S, Liu F, Xiao L, Kanwar YS, Sun L. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biol. 2018 Jun;16:32-46. doi: 10.1016/j.redox.2018.02.013. Epub 2018 Feb 15. Erratum in: Redox Biol. 2019 Jun;24:101216. PMID: 29475133; PMCID: PMC5842313.
    Species: Human/mouse
    Sample type:
  6. Guo W., Liu W., Chen Z., et al. Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis. Nat Commun. 2017 Dec 18;8(1):2168. doi: 10.1038/s41467-017-02351-0.
    Species: Mouse
    Sample type: Conditioned Media
  7. Al-Hakeim H., Al-Rammahi D., Al-Dujaili A. IL-6, IL-18, sIL-2R, and TNF? proinflammatory markers in depression and schizophrenia patients who are free of overt inflammation. J Affect Disord. 2015 Aug 15;182:106-14. doi: 10.1016/j.jad.2015.04.044
    Species: Human
    Sample type: Serum
  8. Hristova J., Dimitrova A., Tsaryanski G., et al. NGAL, IL-18, and KIM-1 in urine for early assessment of the risk of developing acute kidney injury in patients undergoing cardiopulmonary bypass. Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 3(6) pp. 228-232, June, 2015
    Species: Human
    Sample type: Urine
  9. Wadood S., Shawk R., Hashem R. Variants of MMP-9 and TIMP-1 Levels Could be a Predictor of an Early Development of Cardiovascular Diseases in Type 2 Diabetes among Iraqi Patients. Iraqi Journal of Science, 2015, Vol 56, No.1C, pp:622-632
    Species: Human
    Sample type: Serum
  10. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]
    Species: Human
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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