Human IL-2 ELISA

RayBio® Human IL-2 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Assay Format

Detection Range:
4 pg/ml - 1500 pg/ml
Recommended Dilution (serum & plasma):
2 fold
4 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P60568
Gene ID:
  • 3558
Gene Symbols:
  • IL2
Protein Name & Synonyms:
Interleukin-2 (IL-2) (T-cell growth factor) (TCGF) (Aldesleukin)
Target Species:
  • Human

Target Background

IL-2 is a 15 kDa glycoprotein that is produced by helper T cells to promote immunostimulatory or immunoinhibitory activity depending on target cells. It can activate the cytotoxic function of natural killer cells, T lymphocytes and monocytes to enhance the cytotoxicity of these effector cells. Once secreted IL-2 will bind to neighboring cells that harbor its receptor, IL-2R. The IL-2 receptors couples to JAK tyrosine kinases and activates the STAT5 transcription factors. IL-2 therapy has shown to be effective in patients with autoimmune diseases.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Zhao J., Lu D., Moya S., et al. Bispecific T-cell engager (BiTE) immunotherapy of ovarian cancer based on MIL-88A MOF/MC gene delivery system. Applied Materials Today. Volume 20, September 2020, 100701
    Species: Human
    Sample type: Conditioned Media (T cells stimulatied with anti-CD3 and anti-Epcam BITE)
  2. Hatlapatka, Tim, et al. "Optimization of culture conditions for the expansion of umbilical cord-derived mesenchymal stem or stromal cell-like cells using xeno-free culture conditions." Tissue engineering part c: methods 17.4 (2011): 485-493.
    Species: Human
    Sample type: Conditioned Media
  3. Shaker MJ., et al. Immunological Study of Women Infected with Trichomonas vaginalis Parasite in Baghdad city. International Journal of Science and Research. Volume 3 Issue 9, September 2014,
    Species: Human
    Sample type: Serum
  4. Al-Hakeim H., Al-Rammahi D., Al-Dujaili A. IL-6, IL-18, sIL-2R, and TNF? proinflammatory markers in depression and schizophrenia patients who are free of overt inflammation. J Affect Disord. 2015 Aug 15;182:106-14. doi: 10.1016/j.jad.2015.04.044
    Species: Human
    Sample type: Serum (Depressed Patients)
  5. Cervia, Davide, et al. "The protein pheromone Er-1 of the ciliate Euplotes raikovi stimulates human T-cell activity: involvement of interleukin-2 system." Experimental cell research 319.3 (2013): 56-67.
    Species: Human
    Sample type: Conditioned Media (jurkat cells)
  6. Zha X., Chen S., Yang L., et al. Upregulated TCR? Enhances Interleukin-2 Production in T-Cells from Patients with CML. DNA Cell Biol. 2012 Nov; 31(11): 1628-1635. doi: 10.1089/dna.2012.1798
    Species: Human
    Sample type: Conditioned Media (PBMCs with a plasmid IRES Site)
  7. Meltzer K., Standley P., Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion. J Am Osteopath Assoc December 1, 2007 vol. 107 no. 12 527-536
    Species: Human
    Sample type: (Human Primary Cultured Fibroblasts)
  8. Abdullah, Ghaidaa A., and Nadham K. Mahdi. "The Role of Cytokines among Women with Spontaneous Miscarriage." Medical Journal of Islamic World Academy of Sciences 21.3 (2013): 119-124.
    Species: Human
    Sample type: Serum
Used for detection in human serum samples in a cohort of patients experience depression. Great results and simple protocol!

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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