Human IL-4 ELISA

RayBio® Human IL-4 ELISA Kit for cell culture supernatants, plasma, and serum samples.

$402.00  
ELH-IL4-1
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Antigen Information

Accession Number:
  • P05112
Gene ID:
  • 3565
Gene Symbols:
  • IL4
Protein Name & Synonyms:
Interleukin-4 (IL-4) (B-cell stimulatory factor 1) (BSF-1) (Binetrakin) (Lymphocyte stimulatory factor 1) (Pitrakinra)
Target Species:
  • Human

Assay Format

Detection Range:
5 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Abdullah, Ghaidaa A., and Nadham K. Mahdi. "The Role of Cytokines among Women with Spontaneous Miscarriage."?Medical Journal of Islamic World Academy of Sciences?21.3 (2013): 119-124.
    Species: Human
    Sample type: Serum
  2. Franova, S., et al. "Experimental model of allergic asthma."?Respiratory Regulation-The Molecular Approach. Springer, Dordrecht, 2013. 49-55.
    Species: Human
    Sample type: BAL fluid
  3. Barderas R., Bartolome R., Fernandez-Acenero MJ., et al. High expression of IL-13 receptor ?2 in colorectal cancer is associated with invasion, liver metastasis, and poor prognosis.. Cancer Res. 2012 Jun 1;72(11):2780-90. doi: 10.1158/0008-5472.CAN-11-4090
    Species: Human
    Sample type: Conditioned Media
  4. Kirmaz, Cengiz, et al. "Effects of allergen-specific immunotherapy on functions of helper and regulatory T cells in patients with seasonal allergic rhinitis."?European cytokine network?22.1 (2011): 15-23.
    Species: Human
    Sample type: nasal lavage fluid
  5. Boskabady, Mohammad Hossein, et al. "The effect of Nigella sativa alone, and in combination with dexamethasone, on tracheal muscle responsiveness and lung inflammation in sulfur mustard exposed guinea pigs."?Journal of ethnopharmacology?137.2 (2011): 1028-1034.
    Species: Guinnea Pig
    Sample type: Serum
  6. Meltzer K., Standley P., Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion. J Am Osteopath Assoc December 1, 2007 vol. 107 no. 12 527-536
    Species: Human
    Sample type:
  7. Wu S-J, No L-T. Tetrandrine Inhibits Proinflammatory Cytokines, iNOS and COX-2 Expression in Human Monocytic Cells. Biol Pharm Bull. 2007;30:59-62.
    Species: Human
    Sample type: Conditioned Media
We ordered two of these kits and they arrived in good condition and good packaging. The minimum detectable dose of this kit is 5 pg/mL, which is pretty good! The protocol demands lot of washing steps, but that is necessary. Overall, I would recommend this product.

New York Medical College
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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