Human IL-6 ELISA

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ELH-IL6-1

RayBio® Human IL-6 ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Antigen Information

Accession Number:
  • P05231
Gene ID:
  • 3569
Gene Symbol:
  • IFNB2
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2)
Target Species:
  • Human

Assay Format

Detection Range:
3 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Siqi Feng, Ya Su, Li Luo, Jing Fengchuan, Qijian Yi, Serum levels of C1q/tumor necrosis factor related protein-1 in children with Kawasaki disease, Pediatric Research accepted article preview 23 January 2018; doi: 10.1038/pr.2018.16.
    Species: Human
    Sample type: Serum
  2. Liu Z., Sun C., Tao R., et al. Pyrroloquinoline Quinone Decelerates Rheumatoid Arthritis Progression by Inhibiting Inflammatory Responses and Joint Destruction via Modulating NF-?B and MAPK Pathways. Inflammation. 2016 Feb;39(1):248-56. doi: 10.1007/s10753-015-0245-7.
    Species: Human
    Sample type: Conditioned Media
  3. Shih K., Janckila A., Lee W., et al. Effects of bariatric weight loss surgery on glucose metabolism, inflammatory cytokines, and serum tartrate-resistant acid phosphatase 5a in obese Chinese adults. Clin Chim Acta. 2016 Jan 30;453:197-202. doi: 10.1016/j.cca.2015.11.004.
    Species: Human
    Sample type: Serum
  4. Kohh B., Nadarajan K., Shim S., et al. Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF?7 cells by reducing FGF4 protein expression. Molecular Medicine Reports 13: 3406-3414, 2016
    Species: Human
    Sample type: Conditioned Media
  5. Huang CJ., Kwok CF., Chou CH., et al. The effect of exercise on lipid profiles and inflammatory markers in lean male adolescents: a prospective interventional study. J Investig Med. 2015 Jan;63(1):29-34. doi: 10.1097/JIM.0000000000000120.
    Species: Human
    Sample type: Serum
  6. Kuntz S., Asseburg H., Dold S., et al. Inhibition of low-grade inflammation by anthocyanins from grape extract in an in vitro epithelial-endothelial co-culture model. Food Funct. 2015 Apr;6(4):1136-49. doi: 10.1039/c4fo00755g.
    Species: Human
    Sample type: Conditioned Media
  7. Perrigue P., Silva M., Warden C., et al. The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines. Mol Cancer Res. 2015 Apr;13(4):636-50. doi: 10.1158/1541-7786.MCR-13-0268
    Species: Human
    Sample type: Conditioned Media
  8. Protti A., Fortunato F., Artoni A., et al. Platelet mitochondrial dysfunction in critically ill patients: comparison between sepsis and cardiogenic shock. Crit Care. 2015 Feb 11;19:39. doi: 10.1186/s13054-015-0762-7.
    Species: Human
    Sample type: Plasma
  9. Filkova M., Vernerova Z., Hulejova H., et al. Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis. Cytokine. 2015 May;73(1):36-43. doi: 10.1016/j.cyto.2015.01.019.
    Species: Human
    Sample type: Conditioned Media
  10. Lira D., Panissa V., Julio U., Franchini E. Differences in metabolic and inflammatory responses in lower and upper body high-intensity intermittent exercise. Eur J Appl Physiol. 2015 Jul;115(7):1467-74. doi: 10.1007/s00421-015-3127-7
    Species: Human
    Sample type: Serum
This kit worked great. As far as ELISAs go this was a simple shake and bake kit with really clear instructions. Worked perfectly to assess concentrations of whole blood LPS stimulated samples at a 1:20 dilution.
Easy to follow and works exactly as expected.
I used it for cell culture supernatant under induced conditions and tested various treatments. It has very high sensitivity and good linearity, it is very helpful.
This kit is really good and the directions are very straightforward. Have purchased 5 of these kits for different cytokines.
This kit worked well for us in cell culture supernatant. This IL-6 ELISA kit has very good sensitivity.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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