Human IL-7 ELISA

RayBio® Human IL-7 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$723.60  
ELH-IL7-2
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Assay Format

Detection Range:
5 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P13232
Gene ID:
  • 3574
Gene Symbols:
  • IL7
Protein Name & Synonyms:
Interleukin-7 (IL-7)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Shen, Anding, et al. "Endothelial cell stimulation overcomes restriction and promotes productive and latent HIV-1 infection of resting CD4+ T cells." Journal of virology 87.17 (2013): 9768-9779.
    Species: Human
    Sample type: Conditioned Media (MCF7 cells - HIV-1 infection of resting CD4+ T cells)
  2. Ragino YI, Oblaukhova VI, Polonskaya YV, Kuzminykh NA, Shcherbakova LV, Kashtanova EV. The Blood Cytokine Profile of Young People with Early Ischemic Heart Disease Comorbid with Abdominal Obesity. J Pers Med. 2020 Aug 13;10(3):87. doi: 10.3390/jpm10030087. PMID: 32823638; PMCID: PMC7565846.
    Species: Human
    Sample type: Serum
  3. Patel E., Okada S., Hachey K., et al. Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation. BMC Immunol. 2012 Aug 16;13:46. doi: 10.1186/1471-2172-13-46.
    Species: Human
    Sample type: Conditioned Media (Bone Marrow CD34+ cells and hematopoetic progenitor cells)
  4. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-?)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." Clinical rheumatology 30.1 (2011): 133-137.
    Species: Human
    Sample type: Serum
  5. Meltzer K., Standley P., Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion. J Am Osteopath Assoc December 1, 2007 vol. 107 no. 12 527-536
    Species: Human
    Sample type: (Human Primary Cultured Fibroblasts)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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