Human IL-8 ELISA

RayBio® Human IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • P10145
Gene ID:
  • 3576
Gene Symbols:
  • CXCL8
  • IL8
Protein Name & Synonyms:
Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I) Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b) IL-8(5-77) IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF) IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV) IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V) IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]
Target Species:
  • Human

Assay Format

Detection Range:
1 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 fold
1 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Vatsya, Shubham, Premashis Kar, and Richa Dewan. "Changes in levels of interleukin-8 in the serum of patients with hepatitis B virus infection in north indian population."?Journal of Clinical and Experimental Hepatology?3.1 (2013): S65.
    Species: Human
    Sample type: Serum
  2. Mahdi, Nadham Kadham, Abbas Fadhel Ghdbhan, and Murtadh Mohammad Saleh. "Pro-inflammatory Cytokines for Evaluation of the Diagnostic Performance for the Urinary Bladder Cancer."?Austral-Asian Journal of Cancer?12.3 (2013): 169-173.
    Species: Human
    Sample type: Serum
  3. Alba G, Reyes ME, Santa-MarÃ?a C, et al. Transcription of Liver X Receptor Is Down-Regulated by 15-Deoxy-?12,14-Prostaglandin J2 through Oxidative Stress in Human Neutrophils. Bozza PT, ed. PLoS ONE 2012;7(10):e42195. doi:10.1371/journal.pone.0042195.
    Species: Human
    Sample type: Conditioned Media
  4. Chen YL., Huang KF., Kuo WC., et al. Inhibition of glutaminyl cyclase attenuates cell migration modulated by monocyte chemoattractant proteins. Biochem. J. (2012) 442, 403-412. doi:10.1042/BJ20110535
    Species: Human
    Sample type: Conditioned Media
  5. de Matos Cavalcante AG., de Bruin PF., de Bruin VM., et al. Melatonin reduces lung oxidative stress in patients with chronic obstructive pulmonary disease: a randomized, double-blind, placebo-controlled study. J Pineal Res. 2012 Oct;53(3):238-44. doi: 10.1111/j.1600-079X.2012.00992.x
    Species: Human
    Sample type: Exhaled Breath Condensate
  6. Kraus D, Winter J, Jepsen S, JÃ?ger A, Meyer R, Deschner J. Interactions of Adiponectin and Lipopolysaccharide from Porphyromonas gingivalis on Human Oral Epithelial Cells. Heimesaat MM, ed. PLoS ONE 2012;7(2):e30716. doi:10.1371/journal.pone.0030716.
    Species: Human
    Sample type: Conditioned Media
  7. LaValle CR, Zhang L, Xu S, Eiseman JL, Wang QJ. Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer. Molecular Cancer Therapeutics 2012;11(7):1389-1399. doi:10.1158/1535-7163.MCT-11-0887.
    Species: Human
    Sample type: Conditioned Media
  8. Wang XS, Williams LA, Krishnan S, et al. Serum sTNFR1, IL6, and the Development of Fatigue in Patients with Gastrointestinal Cancer Undergoing Chemoradiation Therapy.?Brain, Behavior, and Immunity. 2012;26(5):699-705. doi:10.1016/j.bbi.2011.12.007.
    Species: Human
    Sample type: Serum
  9. Zhao D., Najbauer J., Annala AJ., et al. Human neural stem cell tropism to metastatic breast cancer. Stem Cells. 2012 Feb;30(2):314-25. doi: 10.1002/stem.784.
    Species: Human
    Sample type: Conditioned Media
  10. Raychaudhuri S., Raychauduri S., Genovese M. IL-17 receptor and its functional significance in psoriatic arthritis. Mol Cell Biochem. 2012 Jan;359(1-2):419-29. doi: 10.1007/s11010-011-1036-6.
    Species: Human
    Sample type: Conditioned Media
I ordered this kit for many studies and it works very good for me. I tried different dilution factors.

Florida A&M University
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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