Human IL-8 ELISA

RayBio® Human IL-8 (CXCL8) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Old price: $723.60
$578.88  
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ELH-IL8-CL-2
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Assay Format

Detection Range:
1 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P10145
Gene ID:
  • 3576
Gene Symbols:
  • CXCL8
  • IL8
Protein Name & Synonyms:
Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I) Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b) IL-8(5-77) IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF) IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV) IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V) IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]
Target Species:
  • Human

Target Background

Interleukin 8 (IL-8) is a chemotactic factor that attracts neutrophils, basophils and T-cells during an inflammatory process. It is released from a variety cell types such as monocytes, macrophages, neutrophils and intestine, kidney, placenta and bone marrow cells in response to inflammation. It is involved many cellular processes including cell proliferation, tissue remodeling and angiogenesis. IL-8 mediates its effects by binding to two heterotrimeric G protein-coupled receptors, CXCR1 and CXCR2. Given that it is a major mediator of inflammation, IL-8 was markedly increased in infants diagnosed with perinatal infection.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Kormann, Michael SD, et al. "Expression of therapeutic proteins after delivery of chemically modified mRNA in mice." Nature biotechnology 29.2 (2011): 154-157.
    Species: Human
    Sample type: Conditioned Media (Human and mouse alveolar type II epithelial cell lines, A549 and MLE12)
  2. Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
    Species: Human
    Sample type: Conditioned Media (HUVEC cells in vascular injury model)
  3. Lu H., et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nature Cell Biology (2014) doi:10.1038/ncb3041
    Species: Human
    Sample type: Conditioned Media (Mammary Epithelial Cell lines)
  4. Lippert E., Falk W., Bataille F., et al. Soluble galectin-3 is a strong, colonic epithelial-cell-derived, lamina propria fibroblast-stimulating factor. Gut . 2007 Jan;56(1):43-51. doi: 10.1136/gut.2005.081646. Epub 2006 May 18.
    Species: Human
    Sample type: Conditioned Media (Colonic Epithelial Cells')
  5. Liu B, Liu Z, Chen S, Ki M, Erickson C, Reis-Filho JS, Durham BH, Chang Q, de Stanchina E, Sun Y, Rabadan R, Abdel-Wahab O, Chandarlapaty S. Mutant SF3B1 promotes AKT- and NF-?B-driven mammary tumorigenesis. J Clin Invest. 2021 Jan 4;131(1):e138315. doi: 10.1172/JCI138315. PMID: 33031100; PMCID: PMC7773370.
    Species: Human
    Sample type: Conditioned media (Cell culture supernatants)
  6. de Matos Cavalcante AG., de Bruin PF., de Bruin VM., et al. Melatonin reduces lung oxidative stress in patients with chronic obstructive pulmonary disease: a randomized, double-blind, placebo-controlled study. J Pineal Res. 2012 Oct;53(3):238-44. doi: 10.1111/j.1600-079X.2012.00992.x
    Species: Human
    Sample type: Exhaled Breath Condensate (Exhaled Breath Condensate)
  7. Fernando, Romaine I., et al. "IL-8 signaling plays a critical role in the epithelial–mesenchymal transition of human carcinoma cells." Cancer research 71.15 (2011): 5296-5306.
    Species: Human
    Sample type: Conditioned Media ( MCF7 and PANC-1 tumor cell pairs)
  8. Sanmamed M., et al. Serum interleukin-8 reflects tumor burden and treatment response across malignancies of multiple tissue origins. Clinical Cancer Research, Sept 2014, epub ahead of print.
    Species: Human
    Sample type: Serum
  9. Duckworth C., Zhang L., Carroll S., et al. Overexpression of GAB2 in ovarian cancer cells promotes tumor growth and angiogenesis by upregulating chemokine expression. Oncogene. 2016 Aug 4;35(31):4036-47. doi: 10.1038/onc.2015.472.
    Species: Human
    Sample type: Conditioned Media (Fallopian Tube Secretory Epithelial Cells)
  10. Lo MC., et al. Role of MIF/CXCL8/CXCR2 signaling in the growth of nasopharyngeal carcinoma tumor spheres. Cancer Lett. 2013 Jul 10;335(1):81-92. doi: 10.1016/j.canlet.2013.01.052.
    Species: Human
    Sample type:
It was a convenient assay to follow, and showed accuracy in the triplicates.

University of Connecticut
Human Cell Culture SupernatantsCell type was used.

NanoBST
Great kit, one of the standards in our lab. Easy to use, and customer service is great.

UTSW
I ordered this kit for many studies and it works very good for me. I tried different dilution factors.

Florida A&M University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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