Human IL-6 ELISA

RayBio® Human IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.


Antigen Information

Accession Number:
  • P05231
Gene ID:
  • 3569
Gene Symbols:
  • IFNB2
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2)
Target Species:
  • Human

Assay Format

Detection Range:
3 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
3 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Elsaid A., et al. Association of polymorphisms G(-174)C in IL-6 gene and G(-1082)A in IL-10 gene with tranditional cardiovascular risk factors in patients with coronary artery disease. Indian Journal of Biochemistry and Biophysics. Vol 51, August 2014, pp 282-292
    Species: Human
    Sample type: Serum
  2. Gao, S., Dine, J., et al. Anti-inflammatory and Anti-apoptotic Effect of Combined Treatment with Methylprednisolone and Amniotic Membrane Mesenchymal Stem Cells After Spinal Cord Injury in Rats. Neurochemical Research 2014. Epub Ahead of Print DOI:10.1007/s11064-014-1344-9
    Species: Human
    Sample type: Tissue Lysate
  3. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 [Epub ahead of print]
    Species: Human
    Sample type: Plasma
  4. Gotoh M., et al. Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh et al. Molecular Pain 2014, 10:52. doi:10.1186/1744-8069-10-52
    Species: Human
    Sample type: Conditioned Media
  5. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  6. Lakshmi J., et al. Economical Production of Recombinant Human Interleukin-17E using Industrially Important Salt Inducible Escherichia coli GJ1158: A Fermentation Approach. International Journal of Advanced Science and Technology Vol.68 (2014), pp.23-36.
    Species: Human
    Sample type: Conditioned Media
  7. Lakshmi J., Kotra S., Peravali J., Kumar P
    Species: Human
    Sample type: Cell Lysate
  8. Lu H., et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nature Cell Biology (2014) doi:10.1038/ncb3041
    Species: Human
    Sample type: Conditioned Media
  9. Rattray B., Steel J., Semple S., Ramirez R., Abhayaratna WP. Short-term eccentric exercise in newly diagnosed type II diabetics: an exploratory study. Sport Sciences for Health, June 2014 Epub ahead of print
    Species: Human
    Sample type: Plasma
  10. Santarelli E., Gonnella R., Giovenale G., Cuomo L., Capobianchi A., et al. STAT3 activation by KSHV correlates with IL-10, IL-6 and IL-23 release and an autophagic block in dendritic cells. Scientific Reports, 2014.4 article 4241, doi:10.1038/srep04241
    Species: Human
    Sample type: Conditioned Media
I used this kit for the first time. The protocol is very easy and you'll get a strong result using this kit.

Cedars-Sinai Medical Center
I used this kit to validate results from cytokine arrays. Easy to follow protocol. Great results!!!

Florida A&M University
This Human IL-6 ELISA is a great purchase and product. The standard curve is very nice and linear allowing for easy quantification of your samples. I would highly recommend the kit to anyone studying IL-6 secretion.

Northwestern University
Easy to use and results in one day!

Lankenau Institute for Medical Research
I prefer RayBio ELISA kits for all my cytokine analysis. Pretty good in quality of sample prep. Reliable and consistent results throughout various time periods.

University of Texas Dallas
Measured IL-6 in cell culture supernatant. Easy to follow protocol.

University of Houston
This is our lab's go to kit for measuring IL-6 following immune stimulation. Works great and really simple to prep.
This kit worked well for us and the directions were very straightforward. I used it for cell culture supernatant. I found this kit to be very sensitive.

North Dakota State University
This kit worked great. As far as ELISAs go this was a simple shake and bake kit with really clear instructions. Worked perfectly to assess concentrations of whole blood LPS stimulated samples at a 1:20 dilution.

University of Northern Colorado
Easy to follow and works exactly as expected.

Biocogent, LLC
I used it for cell culture supernatant under induced conditions and tested various treatments. It has very high sensitivity and good linearity, it is very helpful.

University of Illinois at Chicago
This kit is really good and the directions are very straightforward. Have purchased 5 of these kits for different cytokines.

This kit worked well for us in cell culture supernatant. This IL-6 ELISA kit has very good sensitivity.
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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