Human Immune Checkpoint Array C3

$284.00
AAH-ICM-3-2

RayBio® C-Series Human Immune Checkpoint Antibody Array 3 Kit. Detects 12 Human molecules expressed in APCs. Suitable for all liquid sample types.

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Antigen Information

Gene Symbol:
CD200 | CD274 | CD276 | CD70 | CD80 | CD86 | ICOSLG | PDCD1LG2 | TNFRSF14 | TNFRSF9   View more

Assay Format

Number of Targets Detected:
12
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
B7-H2
(ICOS Ligand)
B7-H3
(CD276)
B7-H4
  • Gene Symbol: VTCN1 B7H4 UNQ659/PRO1291
  • Gene ID: 79679
  • Accession #: Q7Z7D3
CD200
HVEM
(TNFRSF14/CD270)
CD27 Ligand
(TNFSF7)
  • Gene Symbol: CD70 CD27L CD27LG TNFSF7
  • Gene ID: 970
  • Accession #: P32970
CD80
(B7-1)
CD86
(B7-2)
OX40 Ligand
(TNFSF4)
B7-H1
(CD274/PD-L1)
PD-L2
(B7-DC)
  • Gene Symbol: PDCD1LG2 B7DC CD273 PDCD1L2 PDL2
  • Gene ID: 80380
  • Accession #: Q9BQ51

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Immune Checkpoint C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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