Human Inflammation Array C3

RayBio® C-Series Human Inflammation Antibody Array 3 Kit. Detects 40 Human Inflammatory Factors. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
CCL1 | CCL11 | CCL15 | CCL2 | CCL24 | CCL3 | CCL4 | CCL5 | CCL8 | CSF1   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-6 R
IL-12 p40
IL-12 p70
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
MIP-1 alpha
MIP-1 beta
MIP-1 delta
TGF beta 1
TNF alpha
TNF beta

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Inflammation Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Carlier, Jérome, et al. "Paracrine inhibition of GM-CSF signaling by human cytomegalovirus in monocytes differentiating to dendritic cells." Blood 118.26 (2011): 6783-6792.
    Species: Human
    Sample type: Conditioned Media (Monocytes were differentiated into DCs)
  2. Drolia R., Tenguria S., Durkes A., et al. Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation. Cell Host Microbe. 2018 Apr 11;23(4):470-484.e7. doi: 10.1016/j.chom.2018.03.004.
    Species: Human
    Sample type: Conditioned Media (Caco-2 cells supernate infected with WT or Lap- strain Listeria)
  3. Zheng Y., Ji S., Wu H., et al. Topical administration of cryopreserved living micronized amnion accelerates wound healing in diabetic mice by modulating local microenvironment. Biomaterials. 2017 Jan;113:56-67. doi: 10.1016/j.biomaterials.2016.10.031.
    Species: Human
    Sample type: Conditioned Media (Living Micronized Amniotic Membrane)
  4. Dumas A., Le-Bury G., Marie-Anais F., et al. The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking. J Cell Biol. 2015 Oct 26; 211(2): 359-372. doi: 10.1083/jcb.201503124
    Species: Human
    Sample type: Conditioned Media (Human primary macrophages infected in vitro with HIV)
  5. Rong Deng , Hai-Liang Zhang , Jun-Hao Huang , Rui-Zhao Cai , Yan Wang , Yu-Hong Chen , Bing-Xin Hu , Zhi-Peng Ye , Zhi-Ling Li , Jia Mai , Yun Huang , Xuan Li , XiaoDan Peng , Gong-Kan Feng , Jun-Dong Li , Jun Tang & Xiao-Feng Zhu (2020): MAPK1/3 kinasedependent ULK1 degradation attenuates mitophagy and promotes breast cancer bone metastasis, Autophagy, DOI: 10.1080/15548627.2020.1850609
    Species: Human
    Sample type: Conditioned media (tumor cell culture media)
  6. Miyoshi N, Oubrahim H, Chock PB, Stadtman ER. Age-dependent cell death and the role of ATP in hydrogen peroxide-induced apoptosis and necrosis. Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1727-31.
    Species: Human
    Sample type: Conditioned Media (Human Fibroblasts treated with H2O2)
  7. Kuo, P. L., et al. "Signalling pathway of isophorone diisocyanate-responsive interleukin-8 in airway smooth muscle cells." European Respiratory Journal37.5 (2011): 1226-1236.
    Species: Human
    Sample type: Conditioned Media (BEAS-2B cells )
  8. He Y., Liu S., Leone S., Newburg DS. Human colostrum oligosaccharides modulate major immunologic pathways of immature human intestine. Mucosal Immunology, 2014, Published online, April 2. doi: 10.1038/mi.2014.20
    Species: Human
    Sample type: Tissue Lysate (Intestinal Tissue)
  9. Rooney P., Srivastava A., Watson L., et al. Hyaluronic acid decreases IL-6 and IL-8 secretion and permeability in an inflammatory model of interstitial cystitis. Acta Biomater. 2015 Jun;19:66-75. doi: 10.1016/j.actbio.2015.02.030.
    Species: Human
    Sample type: Cell Lysate (HTB4 cells)
  10. Chan J., Tan M., Sng M., et al. Cancer-associated fibroblasts enact field cancerization by promoting extratumoral oxidative stress. Cell Death Dis 8 (1), e2562. 2017 Jan 19.
    Species: Human
    Sample type: Conditioned Media (Cancer associated fibroblast cells in squamous cell carcinoma)

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