Human Inflammation Array Q3

Quantibody® Human Inflammation Array 3 Kit. Detects 40 Human Inflammatory Factors. Suitable for all liquid sample types.

$2,981.89  
QAH-INF-3-4
+

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Protein Information

Gene Symbols:
CCL1 | CCL11 | CCL15 | CCL2 | CCL24 | CCL3 | CCL4 | CCL5 | CSF1 | CSF2   View more

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
GCSF
GM-CSF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-15
IL-16
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
MCP-1
(CCL2)
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
PDGF-BB
RANTES
(CCL5)
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Inflammation Array Q3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Nacarelli T., Lau L., Fukumoto T., et al. NAD+ metabolism governs the proinflammatory senescence-associated secretome. Nat Cell Biol . 2019 Mar;21(3):397-407. doi: 10.1038/s41556-019-0287-4. Epub 2019 Feb 18.
    Species: Human
    Sample type: Conditioned Media (IMR90 Fibroblasta and cocultures)
  2. Liu P., Li F., Lin J., et al. m6A-independent genome-wide METTL3 and METTL14 redistribution drives the senescence-associated secretory phenotype. Nature Cell Biology volume 23, pages 355–365 (2021)
    Species: Human
    Sample type: Conditioned Media (IMR90 cells Oncology in vitro culture with RAS induced senescence)
  3. Reategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094
    Species: Human
    Sample type: Conditioned Media (Neutrophil swarming experiments)
  4. Dillman R., Cornforth A., Nistor G., et al. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses. J Immunother Cancer. 2018 Mar 6;6(1):19. doi: 10.1186/s40425-018-0330-1.
    Species: Human
    Sample type: Serum (Serum for melanoma patients undergoing immunotherapy)
  5. Martinaud C., Desterke C., Konopacki J., et al. Osteogenic Potential of Mesenchymal Stromal Cells Contributes to Primary Myelofibrosis. Cancer Res. 2015 Nov 15;75(22):4753-65. doi: 10.1158/0008-5472.CAN-14-3696
    Species: Human
    Sample type: Conditioned Media (Mesencymal Stromal Cells)
  6. Platonov M., Borovjagin AV., Kaverina N., et al. KISS1 tumor suppressor restricts angiogenesis of breast cancer brain metastases and sensitizes them to oncolytic virotherapy in vitro. Cancer Lett. 2018 Mar 28;417:75-88. doi: 10.1016/j.canlet.2017.12.024.
    Species: Human
    Sample type: Conditioned Media (Breast cancer cell lines treated with oncolytic viruses for angiogenic factors)
  7. Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.
    Species: Human
    Sample type: Vitreous Fluid (Vitreous sample from retinopathy patients)
  8. Liao Y., Ivanova L., Zhu H., et al. Cord Blood-Derived Stem Cells Suppress Fibrosis and May Prevent Malignant Progression in Recessive Dystrophic Epidermolysis Bullosa. Stem Cells. 2018 Sep 24. doi: 10.1002/stem.2907.
    Species: Human
    Sample type: Conditioned Media (Umbilical cord stem cells)
  9. Velez G., Roybal C., Colgan D., et al. Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease. JAMA Ophthalmol. 2016 Apr;134(4):444-8. doi: 10.1001/jamaophthalmol.2015.5934.
    Species: Human
    Sample type: Vitreous Fluid (Human Vitreous Fluid samples)
  10. Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.
    Species: Human
    Sample type: Conditioned Media (Embryonic stem cell derived astrocytes in immunodeficient mice)
We used QAH-INF-3 product for plasma samples. Method was very easy to perform as well we used their free scanning service, it was quick.

The technical support is also helpful and quick. Overall we liked product as well service.
We are planning to buy more.

Texas tech university health science cen
This is our third plate. It is a little bit of work, too much washing though, but once you get use of it, it's pretty easy forward. I'd recommend it.

Stanford University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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