Human Insulin ELISA

RayBio® Human Insulin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
4 uIU/ml - 300 uIU/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
4 uIU/ml

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This ELISA kit shows no cross-reactivity with the following cytokines tested: human BDNF, BLC, ENA-78, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P01308
Gene ID:
  • 3630
Gene Symbols:
  • INS
Protein Name & Synonyms:
Insulin [Cleaved into: Insulin B chain Insulin A chain]
Target Species:
  • Human

Target Background

Insulin is a polypeptide hormone, secreted by β-cells in the islets of Langerhans of the pancreas. It plays a crucial role in glucose homeostasis, cell growth and metabolism. Upon secretion, insulin circulates to hepatocytes where glucose is stored in the form of glycogen. Other targets of insulin include skeletal muscles and adipocytes which also take up glucose, thereby reducing blood glucose levels. Insulin triggers glucose uptake, muscle protein synthesis, glycogenesis and lipogenesis via the tyrosine kinase pathway. In addition to its well-studied role in diabetes, insulin acts on key organs in the body, including the brain, heart, kidneys, bone and skin.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ching C., Sun T., Huang W., et al. A circuit design of a low-cost, portable and programmable electroporation device for biomedical applications. Sensors and Actuators B 166- 167 (2012) 292- 300
    Species: Human
    Sample type: Proteins (Trasndermal insulin delivery model)
  2. Uday Chandrika, K., Tripathi, R., Kameshwari, Y. et al. Refunctionalization of Decellularized Organ Scaffold of Pancreas by Recellularization: Whole Organ Regeneration into Functional Pancreas. Tissue Eng Regen Med 18, 99–112 (2021).
    Species: Human
    Sample type: Serum
  3. I. Kaur, N. Bhargavi, K. Kuche, et al., Exploring protein stabilized multiple emulsion with permeation enhancer for oral delivery of insulin, International Journal of Biological Macromolecules (2018)
    Species: Human
    Sample type: Plasma (plasma insulin level)
  4. Agrawal A., Urimi D., Harde H., et al. Folate appended chitosan nanoparticles augment the stability, bioavailability and efficacy of insulin in diabetic rats following oral administration. RSC Adv., 2015,5, 105179-105193 DOI: 10.1039/C5RA19115G
    Species: Human
    Sample type: Plasma (Spprague Dawley rats treated with Insulin nanoparticles)
  5. Rathore S., Bidwai A. Association of Insulin Resistance and Selected Adipokines in Cardiovascular Disease with and without Metabolic Syndrome. JMSCR Volume 2, Issue 5, Pages 1176-1186, May 2014
    Species: Human
    Sample type: Serum
  6. Zhang H, Tong TK, Kong Z, Shi Q, Liu Y, Nie J. Exercise training-induced visceral fat loss in obese women: The role of training intensity and modality. Scand J Med Sci Sports. 2021 Jan;31(1):30-43. doi: 10.1111/sms.13803. Epub 2020 Sep 4. PMID: 32789898.
    Species: Human
    Sample type: Serum
  7. Jorgensen A., Nellemann C., Wohlfahr-Veje C. Interaction between paraoxonase 1 polymorphism and prenatal pesticide exposure on metabolic markers in children using a multiplex approach. Reprod Toxicol. 2014 Nov 22;51C:22-30. doi: 10.1016/j.reprotox.2014.11.005.
    Species: Human
    Sample type: Serum (Pregnant greenhouse workers)
  8. Andersen HR, Wohlfahrt-Veje C, Dalga°rd C, Christiansen L, Main KM, et al. (2012) Paraoxonase 1 Polymorphism and Prenatal Pesticide Exposure Associated with Adverse Cardiovascular Risk Profiles at School Age. PLoS ONE 7(5): e36830. doi:10.1371/journal.pone.0036830
    Species: Human
    Sample type: Serum (Prenatal Pesticide exposure)
  9. Kerem M, Ferahkose Z, Yilmaz U, Pasaoglu H, Ofluoglu E, et al. Adipokines and ghrelin in gastric cancer cachexia.World J Gastroenterol. 2008;14(23):3633-3641.
    Species: Human
    Sample type: Serum
  10. Leisegang K., Bouic P., Menkveld R., Henkel R. Reproductive Biology and Endocrinology 2014, 12:34
    Species: Human
    Sample type: Conditioned Media (Conditioned Media)

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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