Human Insulin Receptor Pathway Phosphorylation Array C1

$251.49
AAH-INSR-1-2

RayBio® C-Series Human Insulin Receptor Pathway Phosphorylation Array 1 Kit. Detects 9 phosphorylated proteins in human samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

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Antigen Information

Gene Symbols:
  • EIF4E
  • FOXO3
  • IGF1R
  • INPP5D
  • INS
  • IRS1
  • PTPN11
  • SHC1
  • STK11

Assay Format

Pathway:
  • Akt Signaling
  • IGF Signaling
  • mTOR Signaling
  • PI3K-AKT Signaling
Number of Targets Detected:
9
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

eIF-4E (P-Ser209)
FOXO-3 (P-Ser413)
IGF-1 R (P-Tyr1165)
Insulin (P-Tyr1189)
IRS-1 (P-Ser318)
  • Gene Symbol: IRS1
  • Gene ID: 3667
  • Accession #: P35568
LKB1 (P-Ser428)
SHC (P-Tyr427)
SHIP1 (P-Tyr1020)
SHP-2 (P-Ser542)
 

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Insulin Receptor Phosphorylation Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Typical Data

Figure 1 - HepG2 or HeLa cells were grown to 80% confluency and then serum starved overnight. HepG2 Cells were treated with IGF for 10 minutes. HeLa Cells were treated with Calyculin A for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Total list price $367.64
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.