Human IP-10 ELISA

RayBio® Human IP-10 (CXCL10) ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Old price: $1,608.00
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ELH-IP10-5
+

Assay Format

Detection Range:
8.23 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
2.5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP- 2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P02778
Gene ID:
  • 3627
Gene Symbols:
  • CXCL10
  • INP10
  • SCYB10
Protein Name & Synonyms:
C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) [Cleaved into: CXCL10(1-73)]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ko TM., Kuo HC., Chang JS., et al. CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease. Circ Res. 2015 Feb 27;116(5):876-83. doi: 10.1161/CIRCRESAHA.116.305834.
    Species: Human
    Sample type: Plasma (Kawasaki's Disease patients)
  2. Gao Q., Camous X., Lu Y., et al. Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies. Neurobiol Aging. 2016 Mar;39:140-6. doi: 10.1016/j.neurobiolaging.2015.12.002.
    Species: Human
    Sample type: Plasma (Cognitive Performance)
  3. Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
    Species: Human
    Sample type: Conditioned Media (HIV innoculated brain periocytes)
  4. Marozin S., Altonmonte J., Munoz-Alvarez K., et al. STAT3 inhibition reduces toxicity of oncolytic VSV and provides a potentially synergistic combination therapy for hepatocellular carcinoma. Cancer Gene Ther. 2015 Jun;22(6):317-25. doi: 10.1038/cgt.2015.23.
    Species: Human
    Sample type: Conditioned Media (Carcinoma Cell lines treated with a Stat3 inhibitor or control)
  5. Bratland, Eirik, Alexander Hellesen, and Eystein S. Husebye. "Induction of CXCL10 chemokine in adrenocortical cells by stimulation through toll-like receptor 3." Molecular and cellular endocrinology 365.1 (2013): 75-83.
    Species: Human
    Sample type: Plasma (autoimmune Addison’s disease patients)
  6. Fujiwara S., Nagai H., Oniki S., et al. Interleukin (IL)-17 versus IL-27: opposite effects on tumor necrosis factor-?-mediated chemokine production in human keratinocytes. Exp Dermatol. 2012 Jan;21(1):70-2. doi: 10.1111/j.1600-0625.2011.01384.x
    Species: Human
    Sample type: Conditioned Media (Normal human epidermal keratinocytes)
  7. Vargas-Inchaustegui D., Hogg A., Tulliano G., et al.CXCL10 Production by Human Monocytes in Response to Leishmania braziliensis Infection. Infect. Immun. January 2010 vol. 78 no. 1 301-308
    Species: Human
    Sample type: Conditioned Media (PBMC and macrophage cultures)
  8. Hardy GAD, Sieg S, Rodriguez B, et al. Interferon-? is the primary plasma type-I IFN in HIV-1 infection and correlates with immune activation and disease markers. PLoS ONE. 2013;8(2):e56527.
    Species: Human
    Sample type: Plasma (detection limit 8.00pg/ml)
  9. Cakir, Mehtap, et al. "Serum CXCL10 levels and neuromuscular manifestations in patients with autoimmune thyroid diseases." Autoimmunity 44.6 (2011): 496-503.
    Species: Human
    Sample type: Serum
  10. Wu C, Lu X, Wang X, et al. Clinical symptoms, immune factors, and molecular characteristics of an adult male in Shenzhen, China infected with influenza virus H5N1. J Med Virol. 2013 May;85(5):760-768.
    Species: Human
    Sample type: Serum
The Human IP-10 ELISA was used to test the levels of secreted C-X-C Motif Chemokine Ligand 10 upon treatment of cancer cells with chemotherapeutics. Results were consistent.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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