770-729-2992

Home/Search results/Products/Arrays/Antibody Arrays/C-Series/JAK/STAT Arrays/Human JAK/STAT Pathway Phosphorylation Array C1

Manual / SDS

A431 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with EGF for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Description
Citations
Reviews
Ask a question
Files

Assay Format

Pathway:

Akt Signaling
HER/ErbB Signaling
JAK/STAT Signaling
MAPK Signaling
Notch Signaling
PI3K-AKT Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
EGFR (P-Ser1070) Gene Symbol: EGFR ERBB ERBB1 HER1 Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human Phospho-EGFR (S1070) ELISA	JAK1 (P-Tyr1022) Gene Symbol: JAK1 JAK1A JAK1B Gene ID: 3716 Accession #: P23458 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Jak1 (Y1022) ELISA	JAK2 (P-Tyr1007/1008) Gene Symbol: JAK2 Gene ID: 3717 Accession #: O60674 ELISA Kit: RayBio Human Phospho-JAK2 (Y1007/1008) ELISA	SHP-1 (P-Ser591) Gene Symbol: PTPN6 HCP PTP1C Gene ID: 5777 Accession #: P29350 ELISA Kit: RayBio Human and Mouse Phospho-SHP1 (Ser591) ELISA	SHP2 (P-Tyr542) Gene Symbol: PTPN11 PTP2C SHPTP2 Gene ID: 5781 Accession #: Q06124 ELISA Kit: RayBio Human/Mouse/Rat Phospho-SHP2 (Y542) ELISA
Src (P-Tyr419) Gene Symbol: SRC SRC1 Gene ID: 6714 Accession #: P12931 ELISA Kit: RayBio Human Phospho-SRC (Y419) ELISA	STAT1 (P-Ser727) Gene Symbol: STAT1 Gene ID: 6772 Accession #: P42224 ELISA Kit: RayBio Human/Mouse Phospho-Stat 1 (S727) ELISA	STAT2 (P-Tyr689) Gene Symbol: STAT2 Gene ID: 6773 Accession #: P52630	STAT3 (P-Tyr705) Gene Symbol: STAT3 APRF Gene ID: 6774 Accession #: P40763 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Stat 3 (Y705) ELISA	STAT5 (P-Tyr694) Gene Symbol: STAT5A STAT5 Gene ID: 6776 Accession #: P42229 ELISA Kit: RayBio Human Phosphotyrosine STAT5 ELISA
STAT6 (P-Tyr641) Gene Symbol: STAT6 Gene ID: 6778 Accession #: P42226 ELISA Kit: RayBio Human Phospho-STAT6 (Y641) ELISA	TYK2 (P-Tyr1054) Gene Symbol: TYK2 Gene ID: 7297 Accession #: P29597 ELISA Kit: RayBio Human and Mouse Phospho-TYK2 (Tyr1054) ELISA

Typical Results

A431 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with EGF for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 1: A431 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with EGF for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human JAK/STAT Pathway Phosporylation Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^¢ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.