Human Kiloplex Quantitative Proteomics Array

Quantibody® Human Kiloplex array. A combination of 25 nonoverlapping arrays. Detects 1000 Human Biomarkers. Suitable for all liquid sample types.

[Price not listed]  
QAH-CAA-X00-1

Antigen Information

Gene Symbols:
ACAN | ACE | ACE2 | ACP5 | ACVR1 | ACVR1B | ACVR2A | ACVR2B | ADA | ADAM12   View more

Assay Format

Number of Targets Detected:
1000
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 1.5 mL
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

17-beta-HSD1 (HSD17B1), 2B4 (CD244), 4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), 5'-Nucleotidase (CD73), 6Ckine (CCL21), ACE, ACE-2, Activin A, Activin R1A (ALK-2), Activin R1B (ALK-4), Activin R2A, Activin R2B (ACVR2B), ADA, ADAM-12, ADAM-22, ADAM23, ADAM-8, ADAM-9, ADAMTS-13, ADAMTSL-1, Adiponectin (ACRP30), Adipsin (Complement Factor D), ADP-Ribosyl Cyclase 2 (BST-1/CD157), aFGF (FGF-1), Aggrecan, AgRP, AIF, AKR1C4, Albumin, ALCAM (CD166), ALK-1, ALK-6 (BMPR-IB), Alpha-fetoprotein, AMICA, AmiGO, AmiGO2, Aminopeptidase LRAP (ERAP2), Aminopeptidase P2 (XPNPEP2), Amnionless, Amphiregulin, AMSH (STAMBP), Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL4, ANGPTL7, Annexin A5 (Annexin V), APRIL, Arginase 1, ARNT (HIF-1 beta), Artemin, Arylsulfatase A (ARSA), Arylsulfatase B (ARSB), ASAH2 (Neutral Ceramidase), ASAHL, AXL, B3GNT2, B4GalT1, B7-H1 (CD274), B7-H2 (ICOS Ligand), B7-H3 (CD276), B7-H4, BAFF, BAFF R, BAI1, BAMBI (NMA), BATF3, BCAM, BCL-10, BCL-2, bcl-w, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), Beta-glucuronidase (GUSB), beta-NGF, bFGF, BID, Biglycan, BLAME (SLAMF8), BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-8, BMP-9, BMPR-IA (ALK-3), BMPR-II, BOC (Brother of CDO), Bora, Brevican, Brorin (VWC2)...View all 1000 human target proteins

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • 25 nonoverlapping Quantibody slides
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Liang Y, Li J, Li Q, Tang L, Chen L, Mao Y, He Q, Yang X, Lei Y, Hong X, Zhao Y, He S, Guo Y, Wang Y, Zhang P, Liu N, Li Y, Ma J. Plasma protein-based signature predicts distant metastasis and induction chemotherapy benefit in Nasopharyngeal Carcinoma. Theranostics. 2020 Aug 1;10(21):9767-9778. doi: 10.7150/thno.47882. PMID: 32863958; PMCID: PMC7449924.
    Species: Human
    Sample type: Plasma
  2. Wang C, Wang Y, Hong T, Ye J, Chu C, Zuo L, Zhang J, Cui X. Targeting a positive regulatory loop in the tumor-macrophage interaction impairs the progression of clear cell renal cell carcinoma. Cell Death Differ. 2020 Oct 2. doi: 10.1038/s41418-020-00626-6. Epub ahead of print. PMID: 33009518.
    Species: Human
    Sample type: Tissue Lysate (ccRCC tissues)
  3. Platonov M., Borovjagin AV., Kaverina N., et al. KISS1 tumor suppressor restricts angiogenesis of breast cancer brain metastases and sensitizes them to oncolytic virotherapy in vitro. Cancer Lett. 2018 Mar 28;417:75-88. doi: 10.1016/j.canlet.2017.12.024.
    Species: Human
    Sample type: Conditioned Media (Breast cancer cell lines treated with oncolytic viruses for angiogenic factors)
  4. Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5.
    Species: Human
    Sample type: Conditioned Media (Embryonic stem cell derived astrocytes in immunodeficient mice)
  5. Leung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327
    Species: Human
    Sample type: Conditioned Media (A375 ERK2 treated cells with or without ddoxycycline)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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