Human L4 Array, Glass Slide

RayBio® L-Series Human Antibody Array 4 Glass Slide Kit. Detects 500 Human Proteins. Suitable for serum, plasma and cell culture supernatants. (4 or 8 Sample Kit)

AAH-BLG-4-4
+

Antigen Information

Gene Symbols:
ABCC1 | ACP1 | ACTG1 | AFF3 | AGRN | AMY1A | ANP32A | AOC3 | AQR | ARHGDIA   View more

Assay Format

Number of Targets Detected:
500
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Label-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit
NEED HELP RUNNING THIS ASSAY? 50% Off Discovery Proteomics Services Through december REQUEST QUOTE | MORE INFO

Product Features

  • High density arrays
  • 500 Human proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Names

HSPA1A, HTRA1, Human Agrin, IBP160 , IDH1, IDH3A, IFRD1 , IMP2, IGFBP-rp1, IGSF4B, Ihh, ILK, Inhibin beta, Integrin b1, Integrin beta 6, Intergrin a6, IQGAP1 , IQGAP2 , IRE1, IRS2, ISOC2, ITGB4BP, ITIH1, ITIH2, ITIH3, ITIH4 a, JAM-A, JARID2, Karyopherin beta 1, Keratin 36, Keratin 38, KHSRP, KIAA0319L, KIAA1468, KIAA1967, KIF5B , Kilon, KLK-B1, KMD4B, KMT2B, KRT31, KRT72, KRT73, KRT82, KRT85 N-terminal, KRTDAP, KRTHA3B, KSR1, LAD, LAF4, LAIR1, LAM b1, LAMA, Lamin A/C, Lamin B1, Lamin B2, Laminin 2 alpha, Laminin b2, Laminin gamma 1, LAMP, LAMP1, LAMP2, LAP3, LASP1, Latent TGF beta bp2, LCAT, LCMT2, LDHA, LDHB, LEDGF, LEKTI, LILRA3, LIMS1, LMAN2, LMW-PTP, LOK, LOX, LOXL1, LRP 4, LTA4H, LTBP4, Lubricin, LUZP1, LYPA1, Lysozyme, MAGI2, MAGP-2, MAN1, MANF, Mannosidase II, MAP1A, MAPRE1, MARCKS, Complement MASP3, MBD2 , MBP, MCAM, Mcl-1, MCM, MCM5 , MCMP2, MDH1, MDH2, ME1, Meprin alpha, Metallothionein, Metavinculin, MFAP4, MFI2, mGLUR5, MGP, Mimecan, MINPP1, MLCK, MMR, MMRN1, MN1, Moesin, MP1, MPCA, Myeloperoxidase, MRP 1, MSH6, mTOR, MUCDHL, Multimerin 2, MyBPC3, MYH2, MYH6, MYH7, MYHC, MYL12B , MYL3, MYO5A, Myoferlin , Myosin 18B, Myotrophin, NABC1, NAGLU, NAP1L1 , NAPRT1, NASP , NCAM2 , Nebulin, Nectin-1, Nectin-3, NEDD8, Neogenin, Nesprin2, Neurabin 1, N-Cadherin, Neurofibromin, Neurogranin, Neuropeptide B, Neuropilin-1, Neurotrimin, NF-M, Nidogen-2, NIT2, NME3, nNOS, Noelin, Non-muscle Actin, Non-muscle Myosin IIA, Notch-2, Notch-2 ICD, NPAS3, NPM1, NQO2, NT5C3, Nucleobindin 1, NUP98, OBCAM, OIT3, Olfactomedin-2, Ornithine Carbamoyltransferase , Orosomucoid 2, ORP150, OSBP1, OSCAR, Oncostatin M R beta, Osteoadherin, Oxytocin-neurophysin 1, p16 ARC , P20Sb3, p23, p39 , P4HB, p73, PA2G4, PABP, PACS1, PARVB, PCBP1, PCBP2, PCCA, PCDH7, PCDX8, PCK2 , PCMT1, PCNA, PCPE-1, Proprotein Convertase 9, PCYOX1, PDE1B, PDIA6, PDLIM1, PDLIM5, PDZD2, PEBP4, PEPD, PER1, Perilipin-3, Perilipin-1 , Periostin, Peroxiredoxin 2, Peroxiredoxin 3, Peroxiredoxin 5, PF4V1, PGAM1, PGAM2, PGD, PGDF, PGK-1, PGLS-C-t, PGM1, PGRPL, PHAP1, Phosphoserine Aminotransferase, PI3-Kinase C2 beta, pIgR, PIK3IP1, PIN, PISD, PKLR, PLA2G1B, Plakophilin 1, Plastin L, PLC-gamma 1 , Pleckstrin, Plectin, Plexin B1, Plexin B2, PLOD1, PLOD2, PLS3, Plxdc2, PNP, POR, PPCS, PPOX , PPP2R1B , PPP2R4, PRCP, PRDM13, PRDX 1, PRELP, PREP, PRG2, Prion protein PrP , Profilin 1, Properdin, Prosaposin, Prostaglandin D Synthase, Proteasome 20S a+b, Proteasome 20S alpha, Proteasome 20S alpha 5, Proteasome 20S b7, Proteasome 26S S5, Proteasome beta 1, Proteasome subunit alpha type 6, Proteasome subunit beta type 2, Proteasome subunit beta type 4, Coagulation Factor XIV, Protein Z, Prouroguanylin, PRSS23, PRSS3, Myeloblastin, PSMA1, PSMA2, PSMA4, PSMA7, PSMB5, PSMC3 , PSMD1, PSMD9 , PTEN, PTK 7, PTMA, PTP gamma, PTP kappa, PTP mu, PTPRS, PTPRZ, PYGL, PZP, QDPR, QPRT, Quiescin Q6, Rab7a, Ran, RanGAP1, RAP1AB, Rbm15, RCL , Reg1A, Reg3A, RHOC, RhoGDI, Ribonuclease A, Ribonuclease Inhibitor, Ribonuclease T2, RKIP, RNA Polymerase II, RNASE4, RNASE6, RPL10, RPL10A , RPL11, RPL12, RPL14 , RPL17, RPL22, RPL5, RPL7A, RPLP0 , RPS10, RPS11, RPS12, RPS19, RPS2, RPS20 , RPS23, RPS25, RPS28, RPS3, RPS5, RREB1, RSU1, S100 A1, S100 A11, S100 A7, S100 A9, S100P, SAA, SAA4a, Salivary alpha amylase, SAMSN1, SBP-1, SBSN, SDF4, SDNSF, SDPR, Secretogranin V, Semaphorin 6B, Semaphorin 7A, Semenogelin I, Semenogelin II, Serpin A11, Serpin A7, Serpin B3, Serpin B6, Serpin B8, Serpin F2, Serpin A10, Serpin B1, Serpin B4, Serpin E2, SerRS, SET, SEZ6L2, SF20, SH3BGRL, SH3BGRL3, SHANK1, SHC1, SHIP, SHMT1, SHP-1, Siglec-1, Siglec-14, SIM2 , SIRP beta 1, Six3, SLC38A10, SLITRK1, SLURP1, SMA, SMC4 , SMPD4, SOD1, SOD2, SOD3, SOD4, Somatostatin, SORD, SorLA, SOX4, SP-D, Spectrin beta-5, SPEN, SPINK7, SPTBN1, Src(1), SREC-II, STAT3, Stathmin 1, Sterol carrier protein 2, STI1, STOM, SUCLG1, SUMO3, Symplekin, SynCAM, Syntaxin 7, TAB182, TAGLN2, Talin1, Talin1&2, TAX1BP3 , TBCA, TCEB2, Tcf20, TCN1, TCP1 eta, Tenascin C, Tenascin X(1), TFF2(1), TGM3, Thioredoxin-1, Thimet Oligopeptidase, Thymosin b10, Titin, TLS, TMEM223, TOB2 , TOP2B, TPM4, Tripeptidyl-peptidase I, Transaldolase 1, Transketolase, Transthyretin, TRAP1, TRAP220, TRF 2, Triosephosphate isomerase, Tropomyosin 3, TRP-1, TRPS1, Trypsinogeb-2, TrypsinPan, Tryptophanyl, TSR2, TUBA6, TWF2, TXNDC4, TXNDC5, TXNRD2, UBE2D3, Ube2L3, UBE2N, Ubiquitin+1, UCH-L1, UFM 1, UGGT, UNC-13 Homolog D, UNC45A, UNC5H4, UPB1 , UQCRB, UQCRH , URB, URB2, UROC1, UROD, URP2, USP14 , USP5 , Uteroglobin, Utrophin, VAP-1, VAP-A, VCP, VDAC1 , Versican isoform V0, Vimentin B, VNN1, VSIG4, WDR1, WISP2, WNK2, YB1 , YY1, ZBTB4, ZC3H4-N-t, ZC3H8, ZDHHC18 , ZNF671, Zyxin

Target Species

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Human L4 Antibody Array Glass Slide(s)
  • Dialysis Vials & Floating Dialysis Rack
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • 20x Wash Buffer 1
  • 20x Wash Buffer 2
  • Cy3-Conjugated Streptavidin
  • Adhesive Plastic Strips
  • Labeling Buffer
  • 2z Cell Lysis Buffer
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4, Na2HPO4, and ddH20
  • 1 ml tube, small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • Pipettors, pipette tips and other common lab consumables
  • Laser scanner for fluorescence detection
  • Aluminum foil

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

No posts found

Write A Review
U.S. Non-U.S.

Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Discovery Proteomics Sale Discovery Proteomics Sale

Amazon Gift Card Program

Related Products Related Products