Human Leptin ELISA

RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.

$402.00  
ELH-Leptin-1
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Antigen Information

Accession Number:
  • P41159
Gene ID:
  • 3952
Gene Symbols:
  • LEP
  • OB
  • OBS
Protein Name & Synonyms:
Leptin (Obese protein) (Obesity factor)
Target Species:
  • Human

Assay Format

Detection Range:
2 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma):
20 - 50 fold
Sensitivity:
2 pg/ml

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Specificity:
The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Bhaktha G., et al. SERUM TNF-? AND LEPTIN LEVELS IN GRADED OBESE DIABETIC WOMEN- FREE FROM MICRO AND MACRO VASCULAR COMPLICATIONS. IJPCBS 2014, 4(3), 648-653
    Species: Human
    Sample type: Serum
  2. Bil-lula I., et al. Infectobesity in the Polish Population - Evaluation of an Association between Adenoviruses Type 5, 31, 36 and Human Obesity. International Journal of Virology and Molecular Biology 2014, 3(1): 1-8. DOI: 10.5923/j.ijvmb.20140301.01
    Species: Human
    Sample type: Plasma
  3. Bil-Lula I., et al. Is There any Link Between Visceral Obesity and Adenovirus Infections in the Polish Population? Adv Clin Exp Med 2014, 23, 3, 415-422
    Species: Human
    Sample type: Plasma
  4. Das P., Bandyopadhyay SK., Biswas S., et al. CORRELATION OF OBESITY AND THE NOVEL ADIPOCYTOKINES, LEPTIN AND ADIPONECTIN WITH INSULIN RESISTANCE IN TYPE 2 DIABETES MELLITUS. Bangladesh J Physiol Pharmacol 2014;30(1):29-32.)
    Species: Human
    Sample type: Serum
  5. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]
    Species: Human
    Sample type: Plasma
  6. Ha AW., et al. Plasma adipocytokines and antioxidants-status in Korean overweight and obese females with dyslipidemia. Nutr Res Pract. 2014 Aug;8(4):417-424. http://dx.doi.org/10.4162/nrp.2014.8.4.417
    Species: Human
    Sample type: Plasma
  7. Jorgensen A., Nellemann C., Wohlfahr-Veje C. Interaction between paraoxonase 1 polymorphism and prenatal pesticide exposure on metabolic markers in children using a multiplex approach. Reprod Toxicol. 2014 Nov 22;51C:22-30. doi: 10.1016/j.reprotox.2014.11.005.
    Species: Human
    Sample type: Serum
  8. Leisegang K., Bouic P., Menkveld R., Henkel R. Reproductive Biology and Endocrinology 2014, 12:34 http://www.rbej.com/content/12/1/34
    Species: Human
    Sample type: Serum
  9. Lin Y., et al. Dietary fiber intake and its association with indicators of adiposity and serum biomarkers in European adolescents: the HELENA study. European Journal of Nutrition August 2014, Epub Ahead of Print. DOI 10.1007/s00394-014-0756-2
    Species: Human
    Sample type: Serum
  10. Rathore S., Bidwai A. Association of Insulin Resistance and Selected Adipokines in Cardiovascular Disease with and without Metabolic Syndrome. JMSCR Volume 2, Issue 5, Pages 1176-1186, May 2014
    Species: Human
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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