Human Leptin ELISA

RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.

$402.00
ELH-Leptin-1
+

Antigen Information

Accession Number:
  • P41159
Gene ID:
  • 3952
Gene Symbols:
  • LEP
  • OB
  • OBS
Protein Name & Synonyms:
Leptin (Obese protein) (Obesity factor)
Target Species:
  • Human

Assay Format

Detection Range:
2 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma):
20 - 50 fold
Sensitivity:
2 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Schneiderman J., Schaefer K., Kolodgie F., et al. Leptin locally synthesized in carotid atherosclerotic plaques could be associated with lesion instability and cerebral emboli. J Am Heart Assoc. 2012 Oct;1(5):e001727. doi: 10.1161/JAHA.112.001727.
    Species: Human
    Sample type: Conditioned Media
  2. Badler M., Reitmayer C., Arndt C., et al. Age dependency of estrogen responsiveness in the uterus and adipose tissue of aromatase-knockout (ArKO) mice. J Steroid Biochem Mol Biol. 2012 Jan;128(1-2):29-37. doi: 10.1016/j.jsbmb.2011.09.009
    Species: Mouse
    Sample type: Plasma
  3. Andersen HR, Wohlfahrt-Veje C, Dalga°rd C, Christiansen L, Main KM, et al. (2012) Paraoxonase 1 Polymorphism and Prenatal Pesticide Exposure Associated with Adverse Cardiovascular Risk Profiles at School Age. PLoS ONE 7(5): e36830. doi:10.1371/journal.pone.0036830
    Species: Human
    Sample type: Serum
  4. Brydon L. Adiposity, leptin and stress reactivity in humans. Biological Psychology. 2011;86(2):114-120. doi:10.1016/j.biopsycho.2010.02.010.
    Species: Human
    Sample type: Plasma
  5. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-?)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." Clinical rheumatology 30.1 (2011): 133-137.
    Species: Human
    Sample type: Serum
  6. Labayen, I., et al. "Association between the FTO rs9939609 polymorphism and leptin in European adolescents: a possible link with energy balance control. The HELENA study." International Journal of Obesity 35.1 (2011): 66-71.
    Species: Human
    Sample type: Serum
  7. Tzschoppe, Anja, et al. "Dexamethasone stimulates the expression of leptin and 11?-HSD2 in primary human placental trophoblastic cells." European Journal of Obstetrics & Gynecology and Reproductive Biology 156.1 (2011): 50-55.
    Species: Human
    Sample type: Conditioned Media
  8. Labayen, Idoia, et al. "The effect of ponderal index at birth on the relationships between common LEP and LEPR polymorphisms and adiposity in adolescents." Obesity 19.10 (2011): 2038-2045.
    Species: Human
    Sample type: Serum
  9. Kang, Hong Soon, et al. "Transcriptional profiling reveals a role for ROR? in regulating gene expression in obesity-associated inflammation and hepatic steatosis." Physiological genomics 43.13 (2011): 818-828.
    Species: Human
    Sample type: Serum
  10. Labayen, Idoia, et al. "Sexual dimorphism in the early life programming of serum leptin levels in European adolescents: the HELENA study." The Journal of Clinical Endocrinology & Metabolism 96.8 (2011): E1330-E1334.
    Species: Human
    Sample type: Serum

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program

Customers Also Bought Customers Also Bought