Human Leptin ELISA

RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.

$402.00  
ELH-Leptin-1
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Antigen Information

Accession Number:
  • P41159
Gene ID:
  • 3952
Gene Symbols:
  • LEP
  • OB
  • OBS
Protein Name & Synonyms:
Leptin (Obese protein) (Obesity factor)
Target Species:
  • Human

Assay Format

Detection Range:
2 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma):
20 - 50 fold
Sensitivity:
2 pg/ml

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Specificity:
The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Labayen, I., et al. "Association between the FTO rs9939609 polymorphism and leptin in European adolescents: a possible link with energy balance control. The HELENA study."?International Journal of Obesity?35.1 (2011): 66-71.
    Species: Human
    Sample type: Serum
  2. Tzschoppe, Anja, et al. "Dexamethasone stimulates the expression of leptin and 11?-HSD2 in primary human placental trophoblastic cells."?European Journal of Obstetrics & Gynecology and Reproductive Biology?156.1 (2011): 50-55.
    Species: Human
    Sample type: Conditioned Media
  3. Labayen, Idoia, et al. "The effect of ponderal index at birth on the relationships between common LEP and LEPR polymorphisms and adiposity in adolescents."?Obesity?19.10 (2011): 2038-2045.
    Species: Human
    Sample type: Serum
  4. Kang, Hong Soon, et al. "Transcriptional profiling reveals a role for ROR? in regulating gene expression in obesity-associated inflammation and hepatic steatosis."?Physiological genomics?43.13 (2011): 818-828.
    Species: Human
    Sample type: Serum
  5. Labayen, Idoia, et al. "Sexual dimorphism in the early life programming of serum leptin levels in European adolescents: the HELENA study."?The Journal of Clinical Endocrinology & Metabolism?96.8 (2011): E1330-E1334.
    Species: Human
    Sample type: Serum
  6. Wood, I. Stuart, Tanya Stezhka, and Paul Trayhurn. "Modulation of adipokine production, glucose uptake and lactate release in human adipocytes by small changes in oxygen tension."?PflÃ?gers Archiv-European Journal of Physiology?462.3 (2011): 469.
    Species: Human
    Sample type: Conditioned Media
  7. Kerem M, Ferahkose Z, Yilmaz U, Pasaoglu H, Ofluoglu E, et al. Adipokines and ghrelin in gastric cancer cachexia.World J Gastroenterol. 2008;14(23):3633-3641.
    Species: Human
    Sample type: Serum
  8. Wang B H, Trayhurn P. Acute and prolonged effects of TNF-? on the expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture. Pflugers Arch - Eur J Physiol. 2006;452:418-427
    Species: Human
    Sample type: Conditioned Media
  9. Aguilar, María J., et al. "Insulin resistance and inflammation markers: correlations in obese adolescents."?Journal of clinical nursing?22.13-14 (2013): 2002-2010.
    Species: Human
    Sample type: Serum
  10. Wood, I. Stuart, Tanya Stezhka, and Paul Trayhurn. "Modulation of adipokine production, glucose uptake and lactate release in human adipocytes by small changes in oxygen tension."?Pflügers Archiv-European Journal of Physiology?462.3 (2011): 469.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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