Human Leptin ELISA

RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • P41159
Gene ID:
  • 3952
Gene Symbols:
  • LEP
  • OB
  • OBS
Protein Name & Synonyms:
Leptin (Obese protein) (Obesity factor)
Target Species:
  • Human

Assay Format

Detection Range:
2 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma):
20 - 50 fold
2 pg/ml

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The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Volberg, Vitaly, et al. "Adiponectin and leptin trajectories in Mexican-American children from birth to 9 years of age."?PLoS One?8.10 (2013): e77964.
    Species: Human
    Sample type: Plasma
  2. Bharti, Pariksha, et al. "Periodontal treatment with topical antibiotics improves glycemic control in association with elevated serum adiponectin in patients with type 2 diabetes mellitus."?Obesity research & clinical practice?7.2 (2013): e129-e138.
    Species: Human
    Sample type: Serum
  3. Al-HADRAAWY, SALEEM. "Estimated of some physiological parameter in patients infected with Entamoeba Histolytica in Al-Najaf Al-Ashraf province, Iraq." (2013).
    Species: Human
    Sample type: Serum
  4. Jimenez-Pavon D., Ortega F., Artero E., et al. Physical activity, fitness, and serum leptin concentrations in adolescents. J Pediatr. 2012 Apr;160(4):598-603.e2. doi: 10.1016/j.jpeds.2011.09.058.
    Species: Human
    Sample type: Serum
  5. Fahlbusch F., Ruebner M., Volkert G
    Species: Human
    Sample type: Conditioned Media
  6. Schneiderman J., Schaefer K., Kolodgie F., et al. Leptin locally synthesized in carotid atherosclerotic plaques could be associated with lesion instability and cerebral emboli. J Am Heart Assoc. 2012 Oct;1(5):e001727. doi: 10.1161/JAHA.112.001727.
    Species: Human
    Sample type: Conditioned Media
  7. Badler M., Reitmayer C., Arndt C., et al. Age dependency of estrogen responsiveness in the uterus and adipose tissue of aromatase-knockout (ArKO) mice. J Steroid Biochem Mol Biol. 2012 Jan;128(1-2):29-37. doi: 10.1016/j.jsbmb.2011.09.009
    Species: Mouse
    Sample type: Plasma
  8. Andersen HR, Wohlfahrt-Veje C, Dalga?rd C, Christiansen L, Main KM, et al. (2012) Paraoxonase 1 Polymorphism and Prenatal Pesticide Exposure Associated with Adverse Cardiovascular Risk Profiles at School Age. PLoS ONE 7(5): e36830. doi:10.1371/journal.pone.0036830
    Species: Human
    Sample type: Serum
  9. Brydon L. Adiposity, leptin and stress reactivity in humans.?Biological Psychology. 2011;86(2):114-120. doi:10.1016/j.biopsycho.2010.02.010.
    Species: Human
    Sample type: Plasma
  10. Cuchacovich, Raquel, et al. "Tumor necrosis factor-alpha (TNF-?)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature."?Clinical rheumatology?30.1 (2011): 133-137.
    Species: Human
    Sample type: Serum

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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