Human Leptin ELISA

RayBio® Human Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.

$1,608.00  
ELH-Leptin-5
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Antigen Information

Accession Number:
  • P41159
Gene ID:
  • 3952
Gene Symbols:
  • LEP
  • OB
  • OBS
Protein Name & Synonyms:
Leptin (Obese protein) (Obesity factor)
Target Species:
  • Human

Assay Format

Detection Range:
2 pg/ml - 400 pg/ml
Recommended Dilution (serum & plasma):
20 - 50 fold
Sensitivity:
2 pg/ml

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Specificity:
The Leptin ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Nobili V., Alisi A., Cutrera R., et al. Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver. Thorax. 2015 Aug;70(8):769-81. doi: 10.1136/thoraxjnl-2015-206782
    Species: Human
    Sample type: Serum (Biomarkers in pediatric non-alcoholic fatty liver patients)
  2. Hoo SP, Loh QL, Yue Z, et al. Preparation of a soft and interconnected macroporous hydroxypropyl cellulose methacrylate scaffold for adipose tissue engineering. J Mater Chem B. 2013 May 20; [Epub ahead of print] doi:10.1039/C3TB00446E
    Species: Human
    Sample type: Conditioned Media (ASCs (adipose-derived stem cells))
  3. Labayen, Idoia, et al. "Sexual dimorphism in the early life programming of serum leptin levels in European adolescents: the HELENA study." The Journal of Clinical Endocrinology & Metabolism 96.8 (2011): E1330-E1334.
    Species: Human
    Sample type: Serum
  4. Labayen, I., et al. "Association between the FTO rs9939609 polymorphism and leptin in European adolescents: a possible link with energy balance control. The HELENA study." International Journal of Obesity 35.1 (2011): 66-71.
    Species: Human
    Sample type: Serum
  5. Lin Y., et al. Dietary fiber intake and its association with indicators of adiposity and serum biomarkers in European adolescents: the HELENA study. European Journal of Nutrition August 2014, Epub Ahead of Print. DOI 10.1007/s00394-014-0756-2
    Species: Human
    Sample type: Serum (HELENA Study)
  6. Schneiderman J., Schaefer K., Kolodgie F., et al. Leptin locally synthesized in carotid atherosclerotic plaques could be associated with lesion instability and cerebral emboli. J Am Heart Assoc. 2012 Oct;1(5):e001727. doi: 10.1161/JAHA.112.001727.
    Species: Human
    Sample type: Conditioned Media (Human Aortic and Saphenous Vein cell cultures treated with TNFa or LTA)
  7. Volberg V., Harley KG., Aguilar RS., et al. Associations between perinatal factors and adiponectin and leptin in 9-year-old Mexican-American children. Pediatr Obes. 2013 Dec;8(6):454-63. doi: 10.1111/j.2047-6310.2012.00127.x.
    Species: Human
    Sample type: Plasma (the standard curve was narrowed)
  8. Badler M., Reitmayer C., Arndt C., et al. Age dependency of estrogen responsiveness in the uterus and adipose tissue of aromatase-knockout (ArKO) mice. J Steroid Biochem Mol Biol. 2012 Jan;128(1-2):29-37. doi: 10.1016/j.jsbmb.2011.09.009
    Species: Mouse
    Sample type: Plasma (Armotase knockout mice)
  9. Rathore S., Bidwai A. Association of Insulin Resistance and Selected Adipokines in Cardiovascular Disease with and without Metabolic Syndrome. JMSCR Volume 2, Issue 5, Pages 1176-1186, May 2014
    Species: Human
    Sample type: Serum
  10. Jimenez-Pavon D., Ortega F., Artero E., et al. Physical activity, fitness, and serum leptin concentrations in adolescents. J Pediatr. 2012 Apr;160(4):598-603.e2. doi: 10.1016/j.jpeds.2011.09.058.
    Species: Human
    Sample type: Serum (Physical activity and leptin levels)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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