Human Lipocalin-2 ELISA

RayBio® Human Lipocalin-2 (NGAL) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
4 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
20 - 200 fold
4 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P80188
Gene ID:
  • 3934
Gene Symbols:
  • HNL
  • LCN2
  • NGAL
Protein Name & Synonyms:
Neutrophil gelatinase-associated lipocalin (NGAL) (25 kDa alpha-2-microglobulin-related subunit of MMP-9) (Lipocalin-2) (Oncogene 24p3) (Siderocalin LCN2) (p25)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Hu Y., Xue J., Yang Y., et al. Lipocalin 2 Upregulation Protects Hepatocytes from IL1-?-Induced Stress. Cell Physiol Biochem. 2015;36(2):753-62. doi: 10.1159/000430135
    Species: Human
    Sample type: Conditioned Media (Huh7 Cells following IL-1b treatment, hepatic cell line)
  2. Wang J., Zhu H., Xue J., et al. Effects of storage conditions on the stability of serum CD163, NGAL, HMGB1 and MIP2. Int J Clin Exp Pathol. 2015 Apr 1;8(4):4099-105. eCollection 2015
    Species: Human
    Sample type: Plasma (Chronic Hepatitis B Patients)
  3. Vajrychova M., Kacerovsky M., Tambor V., et al. Microbial invasion and histological chorioamnionitis upregulate neutrophil-gelatinase associated lipocalin in preterm prelabor rupture of membranes. J Matern Fetal Neonatal Med. 2014 Dec 23:1-10. DOI: 10.3109/14767058.2014.991305
    Species: Human
    Sample type: Amniotic Fluid (Amniotic Fluid)
  4. Al-Refai AA., et al. Urinary Neutrophil Gelatinase Associated Lipocalin as a Marker of Tubular Damage in Type 2 Diabetic Patients with and without Albuminuria. Open Journal of Nephrology, 2014, 4, 37-46.
    Species: Human
    Sample type: Urine
  5. Susianti H., Wijaya J., Rastini A., et al. Urinary Neutrophil Gelatinase-Associated Lipocalin to Monitor Lupus Nephritis Disease Activity. Biomark Insights. 2015; 10: 81-87. Published online 2015 Sep 6. doi: 10.4137/BMI.S27625
    Species: Human
    Sample type: Urine (Lupus Nephritis patients)
  6. Oweis A., Alshelleh S., Saadeh N., Jarrah M. Ibdah R., Alzoubi K. Long-term follow0up of contrast-induced acute kidney injury: a study from a developing country. International Journal of Vascular Medicine. 2020 Dec 21
    Species: Human
    Sample type: Serum

No posts found

Write A Review
U.S. Non-U.S.


This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program