Mouse anti-Human MAP3K1 Antibody

Mouse anti-Human MAP3K1 Antibody

$198.00
188-10551-1
+

Antigen Information

Accession Number:
  • Q13233
Gene ID:
  • 4214
Gene Symbols:
  • MAP3K1
  • MAPKKK1
  • MEKK
  • MEKK1
Protein Name & Synonyms:
Mitogen-activated protein kinase kinase kinase 1 (EC 2.7.11.25) (MAPK/ERK kinase kinase 1) (MEK kinase 1) (MEKK 1)
Target Species:
  • Human

Antibody Specifications

Applications:
Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Immunofluorescence: 1-2ug/ml
Western blot: 0.5-1ug/ml
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Concentration:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Domain/Region/Terminus:
Cytoplasmic
Host Species:
Mouse
Preparation:
A partial recombinant protein (aa 1211-1310) was used as the immunogen for the MAP3K1 antibody.
Purity:
Protein G affinity chromatography
Species Reactivity:
Human. Other species not tested.

Description

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli, including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (MEK kinase- 5). MEK kinase-1 activates the ERK and c-Jun NH2-terminal kinase (JNK) pathways by phosphorylation of MAP2K1 and MAP2K4, and also activates the central protein kinases of the NF-kB pathway, CHUK and IKBKB. Additionally, MEK kinase-1 uses an E3 ligase through its PHD domain, a RING-finger-like structure, to target proteins for degradation through ubiquitination.

Storage

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.