Human/Mouse MAPK Phosphorylation Array

RayBio® C-Series Human/Mouse MAPK Phosphorylation Antibody Array 1 Kit. Detects 17 phosphorylated proteins in human or mouse samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.


Antigen Information

Gene Symbols:
AKT1 | CREB1 | GSK3A | GSK3B | HSPB1 | MAP2K1 | MAP2K3 | MAP2K6 | MAPK1 | MAPK14   View more

Assay Format

  • Akt Signaling
  • HER/ErbB Signaling
  • IGF Signaling
  • JAK/STAT Signaling
  • MAPK Signaling
  • NFkB Signaling
  • PI3K-AKT Signaling
  • Wnt/beta-Catenin Signaling
Number of Targets Detected:
Species Detected:
  • Human
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473)
(PKB (P-Ser473))
CREB (P-Ser133)
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
GSK3a (P-Ser21)
GSK3b (P-Ser9)
HSP27 (P-Ser82)
JNK (P-Thr183)
MEK (P-Ser217/Ser221)
MKK3 (P-Ser189)
MKK6 (P-Ser207)
MSK2 (P-Ser360)
mTOR (P-Ser2448)
p38 (P-Thr180/Tyr182)
P53 (P-Ser15)
P70S6K (P-Thr421/Ser424)
RSK1 (P-Ser380)
RSK2 (P-Ser386)
  • Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
  • Gene ID: 10432
  • Accession #: P51812

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Typical Data

Figure 1 - HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 250 nM PMA for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Figure 2 - HepG2 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 25 ng/mL of recombinant human IL-1β for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
1.Experimental conditions and sample type
a. In vitro: Human breast cancer cell line (hBCC)
b. In vivo: hBCC grew up in nude mouse bone + mouse bone (human/mouse protein mixed)

Pros: pAKT1, pCREB, pGSK3b,p70S6K, pRSK1,2, pMEK1, pp38, and pMKK3,6 are good. The experimental process is very easy. Easy to reproduce the results

Cons: pERK1/2 is a signal relatively weak compared to the Cell signaling antibody.

Yale University
This assay is very straightforward and provides good results for brain samples.

These arrays are what I was looking for. They are easy to use, very clean membranes with super sharp results. These arrays are a great second step for large scale analysis such RNAseq.

The University of Iowa
Easy to use and convenient way to look at multiple targets.

Midwestern University
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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