RayBio® Human MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.


Assay Format

Detection Range:
2 pg/ml - 500 pg/ml
Recommended Dilution (serum & plasma):
2 fold
2 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P13500
Gene ID:
  • 6347
Gene Symbols:
  • CCL2
  • MCP1
  • SCYA2
Protein Name & Synonyms:
C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2)
Target Species:
  • Human

Target Background

The monocyte chemoattractant protein-1 (MCP-1/CCL2) is a potent chemotactic factor for monocytes. MCP-1 is made of 76 amino acids and is 13 kDa in size. It is produced by a variety of different cell types such as, endothelial, fibroblasts, epithelial, smooth muscle, astrocytic, monocytic and microglial cells. MCP-1/CCL2 mediates its effects through the receptor, CCR2. Genetic variants of MCP-1/CCL2 affect serum levels and the incidence of myocardial infarction. Both MCP-1/CCL2 and its receptor have been associated with diseases such as thrombus formation, tuberculosis, cancer, HIV and multiple sclerosis.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
    Species: Human
    Sample type: Conditioned Media (HUVEC cells in vascular injury model)
  2. Tian, Wenfang, et al. "Brahma?related gene 1 bridges epigenetic regulation of proinflammatory cytokine production to steatohepatitis in mice." Hepatology58.2 (2013): 576-588.
    Species: Human
    Sample type: Conditioned Media (HepG2 cells )
  3. Said N., Sanchez-Carbayo M., Smith S., and Theodorescu D.RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. Apr 2, 2012; 122(4): 1503-1518. doi: 10.1172/JCI61392
    Species: Human
    Sample type: Conditioned Media (Human UMUC3 coculture)
  4. Said N., Sanchez-Carbayo M., Smith S., and Theodorescu D.RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. Apr 2, 2012; 122(4): 1503-1518. doi: 10.1172/JCI61392
    Species: Human
    Sample type: Tissue Lysate (Tumor lysate from lungs of baldder cancer)
  5. Said, Neveen, et al. "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer." The Journal of clinical investigation 121.1 (2011): 132-147.
    Species: Human
    Sample type: Conditioned Media
  6. Caspar-Bauguil S., Kolditz C., Lefort C., et al. Fatty acids from fat cell lipolysis do not activate an inflammatory response but are stored as triacylglycerols in adipose tissue macrophages. Diabetologia. 2015 Nov;58(11):2627-36. doi: 10.1007/s00125-015-3719-0.
    Species: Human
    Sample type: Conditioned Media (THP-1 cells with or without multipotent adipose derived stem adipoctyes)
  7. Lv M., et al. miR141-CXCL1-CXCR2 Signaling-Induced Treg Recruitment Regulates Metastases and Survival of Non-Small Cell Lung Cancer. Molecular Cancer Therapeutics. OnlineFirst October 27, 2014; doi: 10.1158/1535-7163.MCT-14-0448
    Species: Human
    Sample type: Conditioned Media (Non-small cell lung cancer)
  8. Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
    Species: Human
    Sample type: Conditioned Media (HIV innoculated brain periocytes)
  9. Wang J., Su X., Yang L., et al. The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery. Int J Cancer. 2016 Jun 1;138(11):2688-99. doi: 10.1002/ijc.29998.
    Species: Human
    Sample type: Serum (Lung Cancer Patients)
  10. Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
    Species: Human
    Sample type: Plasma (Diabetic Patient Samples)
Great kit! Protocol is fairly standard with all Raybiotech kits which makes it simple to work with.

University of Houston
Used for analysis of human urine samples, worked perfectly.

I am extremely happy with the results obtained using this kit. I used it before in many studies to measure the cytokine in the supernatant. You might need to try different concentrations to get the best results.

Florida A&M University
Easy and precise

University of Houston
Easy to follow!

Oregon Health & Science University
Very easy instructions to follow and the kit worked very well.

University of Houston
Easy to follow and works exactly as expected.

Biocogent, LLC
Used for analysis of human serum samples, easy to use, clean definitive results for varied human samples.

Washington University
Write A Review
U.S. Non-U.S.


This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Customers Also Bought Customers Also Bought