Human MCP-1 ELISA

RayBio® Human MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

$402.00  
ELH-MCP1-1
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Antigen Information

Accession Number:
  • P13500
Gene ID:
  • 6347
Gene Symbols:
  • CCL2
  • MCP1
  • SCYA2
Protein Name & Synonyms:
C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2)
Target Species:
  • Human

Assay Format

Detection Range:
2 pg/ml - 500 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wallrapp, Christine, et al. "Cell-based delivery of glucagon-like peptide-1 using encapsulated mesenchymal stem cells." Journal of microencapsulation 30.4 (2013): 315-324.
    Species: Human
    Sample type: Conditioned Media (telomerase immortalized human mesenchymal stem cell (hMSC-TERT) proliferating cells or encapsulated cells)
  2. Ng, Zhi Xiang, et al. "Attenuated levels of pro-inflammatory markers in diabetic retinopathy patients undergoing treatment with antihyperglycemic and antihypertensive drugs." Clinics 68.2 (2013): 185-193.
    Species: Human
    Sample type: Plasma (diabetic retinopathy undergoing treatment with antihyperglycemic and antihypertensive drugs)
  3. Bakry R., Sayed D., Sayed S., et al. The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia. Comp Clin Pathol (2015) 24:1573-1577, DOI 10.1007/s00580-015-2118-8
    Species: Human
    Sample type: Serum (Analgesia breast cancer patients)
  4. Bakry, R., Sayed, D., Sayed, S. et al. The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia, Comp Clin Pathol (2015) 24: 1573. doi:10.1007/s00580-015-2118-8
    Species: Human
    Sample type: Serum (paravertebral analgesia patients)
  5. Wang B H, Trayhurn P. Acute and prolonged effects of TNF-? on the expression and secretion of inflammation-related adipokines by human adipocytes differentiated in culture. Pflugers Arch - Eur J Physiol. 2006;452:418-427
    Species: Human
    Sample type: Conditioned Media (cell culture media)
  6. Ebud El-Aziz SA., et al. Platelet activating factor and monocyte chemoattractant protein-1 in children with refractory epilepsy. Egypt J Pediatr Allergy Immunol 2012;10(1):13-18.
    Species: Human
    Sample type: Serum
  7. Wadood S., Saihood Y., Mohammed Z. Determination of Angiopoietin Like Protein-4 Levels and Some Parameters in Iraqi Patients with Type 2 Diabetes Mellitus. Journal of Al-Nahrain University, Vol.18 (2), June, 2015, pp.33-43
    Species: Human
    Sample type: Plasma (Type 2 Diabetes Iraqi patients)
  8. El Saadany, Hanan, et al. "Biochemical and genetic risk factors for atherosclerosis in systemic lupus erythematosus." The Egyptian Rheumatologist 33.1 (2011): 35-43.
    Species: Human
    Sample type: Serum (atherosclerosis risk in systemic lupus erythematosus patients)
  9. Susianti, Hani, et al. "Kadar Antibodi Anti-dsDNA dan Urine Monocyte Chemoattractant Protein-1 pada Nefritis Lupus." Jurnal Kedokteran Brawijaya 27.2 (2013): 96-101.
    Species: Human
    Sample type: Urine
Used for analysis of human urine samples, worked perfectly.

NephroNet
I am extremely happy with the results obtained using this kit. I used it before in many studies to measure the cytokine in the supernatant. You might need to try different concentrations to get the best results.

Florida A&M University
Easy and precise

University of Houston
Easy to follow!

Oregon Health & Science University
Very easy instructions to follow and the kit worked very well.

University of Houston
Easy to follow and works exactly as expected.

Biocogent, LLC
Used for analysis of human serum samples, easy to use, clean definitive results for varied human samples.

Washington University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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