RayBio® Human MCP-2 (CCL8) ELISA Kit for cell culture supernatants, plasma, and serum samples.


Assay Format

Detection Range:
1.5 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma):
2 - 5 fold
1.5 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P80075
Gene ID:
  • 6355
Gene Symbols:
  • CCL8
  • MCP2
  • SCYA10
  • SCYA8
Protein Name & Synonyms:
C-C motif chemokine 8 (HC14) (Monocyte chemoattractant protein 2) (Monocyte chemotactic protein 2) (MCP-2) (Small-inducible cytokine A8) [Cleaved into: MCP-2(6-76)]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Poole E., et al. Latency-associated viral IL-10 encoded by human cytomegalovirus modulates cellular IL-10 and CCL8 secretion during latent infection through changes in the cellular miRNA, has miR-92a. Journal of Virology, Sept 2014 epub ahead of print. doi:10.1128/JVI.02424-14
    Species: Human
    Sample type: Conditioned Media
  2. Chen YL., Huang KF., Kuo WC., et al. Inhibition of glutaminyl cyclase attenuates cell migration modulated by monocyte chemoattractant proteins. Biochem. J. (2012) 442, 403-412. doi:10.1042/BJ20110535
    Species: Human
    Sample type: Conditioned Media (U937 Cells)
  3. Wang D, Prakash J, Nguyen P, et al. Bone Morphogenetic Protein Signaling in Vascular Disease: ANTI-INFLAMMATORY ACTION THROUGH MYOCARDIN-RELATED TRANSCRIPTION FACTOR A. The Journal of Biological Chemistry 2012;287(33):28067-28077. doi:10.1074/jbc.M112.379487.
    Species: Human
    Sample type: Conditioned Media (Pulmonary artery smooth muscles cell cultures)
  4. Ahrens B., Schulz G., Bellach J., et al. Chemokine levels in serum of children with atopic dermatitis with regard to severity and sensitization status. Pediatr Allergy Immunol. 2015 Nov;26(7):634-40. doi: 10.1111/pai.12431.
    Species: Human
    Sample type: Serum (Atopic Dermatitis and chemockine levels)
  5. Yi, Taolin, et al. "Phosphatase inhibitor, sodium stibogluconate, in combination with interferon (IFN) alpha 2b: phase I trials to identify pharmacodynamic and clinical effects." Oncotarget 2.12 (2011): 1155-1164.
    Species: Human
    Sample type: Serum (patients with metastatic melanoma, soft tissue sarcomas, gastrointestinal stromal tumors, and breast or colorectal carcinomas)
  6. Hamilton SA, Tower CL, Jones RL. Identification of chemokines associated with the recruitment of decidual leukocytes in human labour: potential novel targets for preterm labour. PLoS ONE. 2013;8(2):e56946.
    Species: Human
    Sample type: Tissue Lysate (choriodecidua (decidua))
  7. Liu H, Liu Z, Chen J, et al. Induction of CCL8/MCP-2 by mycobacteria through the activation of TLR2/PI3K/Akt signaling pathway. PLoS ONE. 2013;8(2):e56815.
    Species: Human
    Sample type: Pleural Fluid (Pleural effusions, tuberculosis (TB) and non-TB samples)
  8. Borden, Ernest C., et al. "Gene regulatory and clinical effects of interferon ? in patients with metastatic melanoma: a phase II trial." Journal of Interferon & Cytokine Research 31.5 (2011): 433-440.
    Species: Human
    Sample type: Serum
  9. Petrosino S., Puigdemont A., Della Valle M., et al. Adelmidrol increases the endogenous concentrations of palmitoylethanolamide in canine keratinocytes and down-regulates an inflammatory reaction in an in vitro model of contact allergic dermatitis. Vet J. 2016 Jan;207:85-91. doi: 10.1016/j.tvjl.2015.10.060.
    Species: Human
    Sample type: Conditioned Media (Poly IC stimulated HaCaT cells with or without PEA, human keratinocytes)
  10. Dhir, Varun, et al. "Low serum levels of myeloid progenitor inhibitory factor-1 predict good response to methotrexate in rheumatoid arthritis." ISRN inflammation 2013 (2013).
    Species: Human
    Sample type: Serum

No posts found

Write A Review
U.S. Non-U.S.


This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Recently Viewed Recently Viewed

Customers Also Bought Customers Also Bought