Human Metabolic Pathways Array C1

RayBio® C-Series Human Metabolic Pathways Antibody Array 1. Detects Human protein biomarkers from metabolic pathways. Suitable for all liquid sample types.

$963.00  
AAH-META-1-8
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Assay Format

Number of Targets Detected:
41
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ACP5 | AHCY | ALPL | ANPEP | ARSA | BST1 | CA12 | CA9 | CAT | CD38   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ADP-Ribosyl Cyclase 2
(BST-1/CD157)
AHCY
Alkaline Phosphatase
(ALPL)
Alpha-Galactosidase A
(GLA)
Aminopeptidase N
Arylsulfatase A
(ARSA)
  • Gene Symbol: ARSA
  • Gene ID: 410
  • Accession #: P15289
CA9
CA12
Carnosine Dipeptidase-1
(CNDP1)
Catalase
CD38
CDO
Chitotriosidase
Cytochrome C
  • Gene Symbol: CYCS CYC
  • Gene ID: 54205
  • Accession #: P99999
Dopa Decarboxylase
(DDC)
FOLH1
(PSMA)
  • Gene Symbol: FOLH1 FOLH NAALAD1 PSM PSMA GIG27
  • Gene ID: 2346
  • Accession #: Q04609
GCNT1
GLB1
(Beta-galactosidase)
Glucokinase
(GCK)
GSTP1
HNMT
Iduronate 2-Sulfatase
(IDS)
Kynureninase
MIF
NSE
  • Gene Symbol: ENO2
  • Gene ID: 2026
  • Accession #: P09104
NUDT5
PD-ECGF
(Thymidine Phosphorylase)
PLA2G4A
Sirtuin 1
Sirtuin 2
Sirtuin 3
Sirtuin 5
SMPD1
SPHK1
ST3GAL1
ST6GAL1
ST8SIA1
SULT2B1
Thyroid Peroxidase
TRACP
(PAP/ACP5)
Visfatin
(PBEF1)
       

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Metabolic Pathways Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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