RayBio® Human MIF ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.


Antigen Information

Accession Number:
  • P14174
Gene ID:
  • 4282
Gene Symbols:
  • GLIF
  • MIF
  • MMIF
Protein Name & Synonyms:
Macrophage migration inhibitory factor (MIF) (EC (Glycosylation-inhibiting factor) (GIF) (L-dopachrome isomerase) (L-dopachrome tautomerase) (EC (Phenylpyruvate tautomerase)
Target Species:
  • Human

Assay Format

Detection Range:
6 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
6 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Xue N., Lin J., Xing S., et al. Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA. Cancer Res Treat. 2019 Jan;51(1):378-390. doi: 10.4143/crt.2018.070.
    Species: Human
    Sample type: Plasma
  2. Yildirim N., Dikmen Y., Terek M., et al. Do preoperative serum vascular endothelial growth factor and migration-inhibitory factor predict the nature of the adnexal masses? A prospective-controlled trial. J Obstet Gynaecol. 2016 Jan 13:1-5. Epub ahead of print
    Species: Human
    Sample type: Serum
  3. Brennan-Bourdon L., De La Cruz-Mosso U., Reyes-Castillion Z., et al. MIF and TNF? serum levels in rheumatoid arthritis patients treated with disease-modifying antirheumatic drugs: a cross-sectional study. Immunopharmacol Immunotoxicol. 2015 Apr;37(2):207-13. doi: 10.3109/08923973.2015.1017645.
    Species: Human
    Sample type: Serum
  4. Bakry R., Sayed D., Sayed S., et al. The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia. Comp Clin Pathol (2015) 24:1573-1577, DOI 10.1007/s00580-015-2118-8
    Species: Human
    Sample type: Serum
  5. Bakry, R., Sayed, D., Sayed, S. et al. The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia, Comp Clin Pathol (2015) 24: 1573. doi:10.1007/s00580-015-2118-8
    Species: Human
    Sample type: Serum
  6. Morales-Zambrano R., et al. Macrophage migration inhibitory factor (MIF) promoter polymorphisms (-794 CATT5-8 and -173 G>C): association with MIF and TNF? in psoriatic arthritis. Int J Clin Exp Med 2014;7(9):2605-2614
    Species: Human
    Sample type: Serum
  7. Muller I., et al. Gremlin-1 inhibits macrophage migration inhibitory factor-dependent monocyte function and survival. International Journal of Cardiology, 2014 Epub Ahead of print
    Species: Human
    Sample type: Conditioned Media
  8. Llamas-Covarrubias MA, Valle Y, Bucala R, et al. Macrophage migration inhibitory factor (MIF): Genetic evidence for participation in early onset and early stage rheumatoid arthritis. Cytokine. 2013;61(3):759-765.
    Species: Human
    Sample type: Serum
  9. Çatak, Onur, Orhan Aydemir, and Bilal Üstünda?. "Vernal keratokonjonktivitte gözya?? makrofaj migrasyon inhibitör faktör düzeyleri."?Journal of Clinical and Experimental Investigations?4.2 (2013): 195-198.
    Species: Human
    Sample type: Tears
  10. Yilmaz O., Kucuk M., Kebapcilar L., et al. Macrophage migration-inhibitory factor is elevated in pregnant women with gestational diabetes mellitus. Gynecol Endocrinol. 2012 Jan;28(1):76-9. doi: 10.3109/09513590.2011.588757.
    Species: Human
    Sample type: Serum

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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