Human MIG ELISA

RayBio® Human MIG (CXCL9) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,680.00  
ELH-MIG-5
+

Assay Format

Detection Range:
20 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
20 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q07325
Gene ID:
  • 4283
Gene Symbols:
  • CMK
  • CXCL9
  • MIG
  • SCYB9
Protein Name & Synonyms:
C-X-C motif chemokine 9 (Gamma-interferon-induced monokine) (Monokine induced by interferon-gamma) (HuMIG) (MIG) (Small-inducible cytokine B9)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Bratland, Eirik, Alexander Hellesen, and Eystein S. Husebye. "Induction of CXCL10 chemokine in adrenocortical cells by stimulation through toll-like receptor 3." Molecular and cellular endocrinology 365.1 (2013): 75-83.
    Species: Human
    Sample type: Plasma (autoimmune Addison’s disease patients)
  2. De Muro, Pierina, et al. "Kidney post-transplant monitoring of urinary glycosaminoglycans/proteoglycans and monokine induced by IFN-? (MIG)." Clinical and experimental medicine 13.1 (2013): 59-65.
    Species: Human
    Sample type: Urine (kidney transplant patients with/without rejection)
  3. Hamilton SA, Tower CL, Jones RL. Identification of chemokines associated with the recruitment of decidual leukocytes in human labour: potential novel targets for preterm labour. PLoS ONE. 2013;8(2):e56946.
    Species: Human
    Sample type: Tissue Lysate (choriodecidua (decidua))
  4. Spaks A., Jaunalksne I., Spaka I., et al. Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer. Anticancer Res. 2015 Dec;35(12):6979-83.
    Species: Human
    Sample type: Serum (Non small cell lung cancer patients vs controls)
  5. Kine E., Trine B., Alexander H., et al. Peripheral Blood Cells from Patients with Autoimmune Addison's Disease Poorly Respond to Interferons In Vitro, Despite Elevated Serum Levels of Interferon-Inducible Chemokines. J Interferon Cytokine Res. 2015 Oct;35(10):759-70. doi: 10.1089/jir.2014.0171
    Species: Human
    Sample type: Serum (Adddison disease that poorly respond to IFNs)
  6. Kine E., Trine B., Alexander H., et al. Peripheral Blood Cells from Patients with Autoimmune Addison's Disease Poorly Respond to Interferons In Vitro, Despite Elevated Serum Levels of Interferon-Inducible Chemokines. J Interferon Cytokine Res. 2015 Oct;35(10):759-70. doi: 10.1089/jir.2014.0171
    Species: Human
    Sample type: Plasma (Adddison disease that poorly respond to IFNs)
I used this kit on patient ascites samples. I was pleased with the results. I diluted my samples 2-fold and most samples were higher than the range of the kit. I was able to extrapolate to get my results, though in the future I would dilute my samples more.

University of New Mexico
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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