Human MIP-1 alpha ELISA

RayBio® Human MIP-1 alpha (CCL3) ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$756.00  
ELH-MIP1a-2
+

Assay Format

Detection Range:
6 pg/ml - 3000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
6 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P10147
Gene ID:
  • 6348
Gene Symbols:
  • CCL3
  • G0S19-1
  • MIP1A
  • SCYA3
Protein Name & Synonyms:
C-C motif chemokine 3 (G0/G1 switch regulatory protein 19-1) (Macrophage inflammatory protein 1-alpha) (MIP-1-alpha) (PAT 464.1) (SIS-beta) (Small-inducible cytokine A3) (Tonsillar lymphocyte LD78 alpha protein) [Cleaved into: MIP-1-alpha(4-69) (LD78-alpha(4-69))]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Schepers, Koen, et al. "Myeloproliferative neoplasia remodels the endosteal bone marrow niche into a self-reinforcing leukemic niche." Cell stem cell 13.3 (2013): 285-299.
    Species: Human
    Sample type: Conditioned Media (Myeloid-enriched BM cells)
  2. Wang J., Su X., Yang L., et al. The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery. Int J Cancer. 2016 Jun 1;138(11):2688-99. doi: 10.1002/ijc.29998.
    Species: Human
    Sample type: Serum (Lung Cancer Patients)
  3. Lu P., Zheng D., Fang C., et al. Cytokines in cerebrospinal fluid of neurosyphilis patients: Identification of Urokinase plasminogen activator using antibody microarrays. J Neuroimmunol. 2016 Apr 15;293:39-44. doi: 10.1016/j.jneuroim.2015.12.010.
    Species: Human
    Sample type: CSF (CSF from neurosyphilis patients)
  4. Leow K., Goh W., Heng C. Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages. Inflamm Res. 2012 Apr;61(4):391-8. doi: 10.1007/s00011-011-0424-4.
    Species: Human
    Sample type: Conditioned Media (Human THP-1 macrophage cell lines)
  5. Cai D, Xu Y, Ding R, Qiu K, Zhang R, Wang H, Huang L, Xie X, Yan H, Deng Y, Lin X, Shao J, Luo X, Duan C. Extensive serum biomarker analysis in patients with non-small-cell lung carcinoma. Cytokine. 2020 Feb;126:154868. doi: 10.1016/j.cyto.2019.154868. Epub 2019 Oct 16. PMID: 31629110.
    Species: Human
    Sample type: Serum
  6. Han Z., Li J., Chen Z. Genetic polymorphism of CCL2-2510 and susceptibility to enterovirus 71 encephalitis in a Chinese population. Archives of Virology. 10.1007/s00705-014-2082-7 [Epub ahead of print]
    Species: Human
    Sample type: Plasma (Plasma)
  7. Xue N., Lin J., Xing S., et al. Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA. Cancer Res Treat. 2019 Jan;51(1):378-390. doi: 10.4143/crt.2018.070.
    Species: Human
    Sample type: Plasma (Nasopharyngeal carcinom patients biomarker)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Recently Viewed Recently Viewed

Customers Also Bought Customers Also Bought